Abstract
The functional assay of a eukaryotic mRNA, into which additional AUG codons have been introduced by in vitro mutagenesis, shows that a translational initiation site need not necessarily be the nearest AUG codon to the 5′ end of a mRNA. Hence, the sequence surrounding the AUG, and not simply its position relative to the 5′ end of mRNA, appears to be important in determining initiation efficiency.
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Lomedico, P., McAndrew, S. Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5′ end of mRNA. Nature 299, 221–226 (1982). https://doi.org/10.1038/299221a0
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DOI: https://doi.org/10.1038/299221a0
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