Abstract
The simultaneous and rapid measurement of the amounts of two different fluorochrome-coupled antibodies bound to single cells (two-colour immunofluorescence) provides a very powerful means for the identification of lymphocyte subpopulations1. Using a dual-laser fluorescence-activated cell sorter (FACS) we show that two monoclonal antibodies, anti-IgM and anti-IgD, labelled respectively with fluorescent and ‘Texas red’ (a new red-fluorescent dye) reveal several previously unrecognized B-cell subpopulations in mouse spleen and lymph nodes. Measured individually, these surface markers (IgM and IgD) show only that B cells are broadly heterogeneous with respect to the amount of surface immunoglobulin expressed2; however, measured simultaneously, they clearly define at least two B-cell subsets. One of these populations, which is predominant in spleen and constitutes the overwhelming majority of B cells in lymph nodes, is missing in CBA/N (Xid) mice known to be deficient with respect to their B-cell immune responses3.
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Hardy, R., Hayakawa, K., Haaijman, J. et al. B-cell subpopulations identified by two-colour fluorescence analysis. Nature 297, 589–591 (1982). https://doi.org/10.1038/297589a0
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DOI: https://doi.org/10.1038/297589a0
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