Abstract
High-resolution phosphorous (31P)-NMR spectra of biological molecules provide detailed information about the metabolism of living systems1–4. Although the NMR method is non-destructive, all studies so far, with two exceptions5,6, have been carried out on excised, perfused organs and tissues or have required some form of surgery2 for in situ measurements. The use of ‘Surface’ radiofrequency coils5 does not require surgery but is best suited for tissues close to the surface of the animals. We describe here ‘topical magnetic resonance’—a new, non-surgical method for acquiring 31P-NMR spectra from a selected, localized place deep within an animal by modifying the main magnetic field, B0, using only static-field gradients. The method is conceptually similar to one spin-imaging method7 but primarily provides biochemical rather than spatial information. This new technique can be used in fundamental investigations into living systems, clinical diagnosis and the estimation of the efficacy of drug therapy.
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Gordon, R., Hanley, P., Shaw, D. et al. Localization of metabolites in animals using 31P topical magnetic resonance. Nature 287, 736–738 (1980). https://doi.org/10.1038/287736a0
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DOI: https://doi.org/10.1038/287736a0
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