Abstract
THE genomes of E. coli and some of its plasmids contain a class of elements called insertion sequences (IS)1–5. These elements exhibit various properties associated with modulation and alteration of gene expression. IS1 (length 800 nucleotide pairs2,3,) apparently plays a role in the amplification of resistance determinants in drug resistance factors5,6 and also causes polar insertion mutations2,3. IS2 (length 1,400 nucleotide pairs2) behaves as a controlling element, which can turn gene expression on or off7. IS3 (length 1,400 nucleotide pairs3) has been associated with strong polar mutations, and is a constituent of a transposable drug resistance determinant8. IS2 and IS3 have also been shown to specify integration loci for the fertility plasmid F4,9,10. Hybridisation data have allowed estimation of the number of IS1 and IS2 elements in the E. coli genome1, but except for the region around lac and purE, little is known about their natural location in the genome.
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DEONIER, R., HADLEY, R. Distribution of inverted IS-length sequences in the E. coli K12 genome. Nature 264, 191–193 (1976). https://doi.org/10.1038/264191a0
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DOI: https://doi.org/10.1038/264191a0
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