Abstract
DENSITY-DEPENDENT1 or contact-inhibited2 cells stop dividing when they reach confluency; however, transformed cells do not exhibit this type of growth control3. Several experiments suggest that adenosine 3′,5′-cyclic monophosphate (cyclic AMP) is involved in regulating the cell division of cultured fibroblasts. The dibutyryl derivative of cyclic AMP inhibits proliferation of transformed cells4, and the steady state levels of cyclic AMP in normal density-dependent fibroblasts are higher than the corresponding virus and spontaneous transformed cell lines5,6. There are, however, discrepancies between experiments which have attempted to compare the cyclic AMP levels of logarithmically growing with quiescent, density-inhibited 3T3 cells. Experiments in our laboratory showed that cyclic AMP levels of 3T3 cells do not change over the cell density of 1 × 104 – 6 × 104 cells per cm2 (ref. 5). These results have been confirmed by others using the same cell system7. Otten et al.6,8, however, reported that cyclic AMP levels of logarithmically growing 3T3 cells are lower than those of confluent, quiescent cells; Heidrick and Ryan9 reported similar results using L cells.
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BANNAI, S., SHEPPARD, J. Cyclic AMP, ATP and cell contact. Nature 250, 62–64 (1974). https://doi.org/10.1038/250062a0
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DOI: https://doi.org/10.1038/250062a0
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