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Use of DEAE-‘Sephadex’ for the Fractionation of Allergic Serum

Abstract

RECENTLY, Gelotte et al.1 have reported that chromatography on DEAE-‘Sephadex A-50’ of a 7S fraction of normal human serum resulted in the isolation of IgGSS) and IgA1A) components which appeared to be free of other protein constituents. We have modified their procedure for the isolation of fractions containing skin-sensitizing activity from the sera of ragweed-sensitive individuals.

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References

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GOODFRIEND, L., PERELMUTTER, L. & ROSE, B. Use of DEAE-‘Sephadex’ for the Fractionation of Allergic Serum. Nature 205, 718–719 (1965). https://doi.org/10.1038/205718a0

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