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Cytosolic free calcium mediates red light-induced photomorphogenesis

Nature volume 358, pages 753–755 (1992)Cite this article

Abstract

LIGHT is a primary environmental signal regulating plant growth and form1. The receptor phytochrome responds to red light and induces changes in membrane properties and gene expression2,3 through an unknown transduction pathway which is the subject of intense study. Etiolated wheat leaf protoplasts swell in response to red light4, by a mechanism believed to be similar to that involved in phytochrome-regulated leaf growth and unrolling5–7. Here we report that this physiological response is preceded by a transient increase in free calcium in the cytosol, followed by a decrease to below resting level. The timing of this response varied between protoplasts. Cytosolic calcium transients induced by photolytic release of calcium or inositol 1,4,5-trisphosphate from chemically caged forms8–13 inside these protoplasts resulted in red light-induced increases in protoplast volume being mimicked. Our results support the hypothesis that phytochrome-mediated signals are transduced through calcium and help to resolve a long-standing issue in plant physiology.

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Authors and Affiliations

  1. Molecular Signalling Group, Institute of Cell and Molecular Biology, University of Edinburgh, Rutherford Building, Edinburgh, EH9 3JH, UK

    P. S. Shacklock, N. D. Read & A. J. Trewavas

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  1. P. S. Shacklock
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  2. N. D. Read
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  3. A. J. Trewavas
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Shacklock, P., Read, N. & Trewavas, A. Cytosolic free calcium mediates red light-induced photomorphogenesis. Nature 358, 753–755 (1992). https://doi.org/10.1038/358753a0

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