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Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis

Nature volume 351, pages 583–586 (1991)Cite this article

Abstract

DYNAMIN was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,0001. It was found to cross-link microtubules into highly ordered bundles, and appeared to have a role in intermicrotubule sliding in vitro. Cloning and sequencing of rat brain dynamin complementary DNA identified an N-terminal region of about 300 amino acids which contained the three consensus elements characteristic of GTP-binding proteins2. Extensive homology was found between this domain and the mammalian MX proteins which are involved in interferon-induced viral resistance3,4, and with the product of the VPS1 locus in Saccharomyces cerevisiae, which has been implicated both in membrane protein sorting5, and in meiotic spindle pole separation6. Dynamin-containing microtubule bundles were not observed in an immunofluorescence study of cultured mammalian cells7, but a role for a GTP-requiring protein in intermicrotubule sliding during mitosis in plants has been reported8. We report here that Drosophila melanogaster contains multiple tissue-specific and developmentally-regulated forms of dynamin, which are products of the shibire locus previously implicated in endocytic protein sorting9,10.

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References

  1. Shpetner, H. S. & Vallee, R. B. Cell 59, 421–432 (1989).

    Article  CAS  Google Scholar 

  2. Obar, R., Collins, C. A., Hammarback, J. A., Shpetner, H. S. & Vallee, R. B. Nature 347, 256–261 (1990).

    Article  ADS  CAS  Google Scholar 

  3. Staeheli, P., Haller, O., Boll, W., Lindenmann, J. & Weissmann, C. Cell 44, 147–158 (1986).

    Article  CAS  Google Scholar 

  4. Staeheli, P. & Sutcliffe, J. G. Molec. cell. Biol. 8, 4524–4528 (1988).

    Article  CAS  Google Scholar 

  5. Rothman, J. H., Raymond, C. K., Gilbert, T., O'Hara, P. J. & Stevens, T. H. Cell 61, 1063–1074 (1990).

    Article  CAS  Google Scholar 

  6. Yen, E., Driscoll, R., Coltrera, M., Olins, A. & Bloom, K. Nature 349, 713–715 (1991).

    Article  ADS  Google Scholar 

  7. Scaife, R. & Margolis, R. L. J. Cell Biol. 111, 3023–3033 (1990).

    Article  CAS  Google Scholar 

  8. Asada, T., Sonobe, S. & Shibaoka, H. Nature 350, 238–241 (1991).

    Article  ADS  CAS  Google Scholar 

  9. Poodry, C. A. & Edgar, L. J. Cell Biol. 81, 520–527 (1979).

    Article  CAS  Google Scholar 

  10. Kosada, T. & Ikeda, K. J. Cell Biol. 97, 499–507 (1983).

    Article  Google Scholar 

  11. Itoh, N. et al. Proc. natn. Acad. Sci. U.S.A. 83, 4081–4085 (1986).

    Article  ADS  CAS  Google Scholar 

  12. Grigliatti, T. A., Hall, L., Rosenbluth, R. & Suzuki, D. T. Molec. gen. Genet. 120, 107–114 (1973).

    Article  CAS  Google Scholar 

  13. Poodry, C. A. Drosophila Information Service 68, 207–208 (1990).

    Google Scholar 

  14. Poodry, C. A. Devl Biol. 138, 464–472 (1990).

    Article  CAS  Google Scholar 

  15. Herman, B. & Albertini, D. F. J. Cell Biol. 98, 565–576 (1984).

    Article  CAS  Google Scholar 

  16. Matteoni, R. & Kreis, T. E. J. Cell Biol. 105, 1253–1265 (1987).

    Article  CAS  Google Scholar 

  17. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  18. Bloom, G. S. & Vallee, R. B. J. Cell Biol. 96, 1523–1531 (1983).

    Article  CAS  Google Scholar 

  19. Vallee, R. B. Meth. Enzym. 134, 104–116 (1986).

    Article  CAS  Google Scholar 

  20. Kozak, M. Nucleic Acids Res. 15, 8125–8148 (1987).

    Article  CAS  Google Scholar 

  21. Cavener, D. R. Nucleic Acids Res. 15, 1353–1361 (1987).

    Article  CAS  Google Scholar 

  22. Chomczynski, P. & Sacchi, N. Analyt. Biochem. 162, 156–159 (1987).

    Article  CAS  Google Scholar 

  23. Gregory, R. J., Kammermeyer, K. L., Vincent, W. S. & Wadsworth, S. C. Molec. cell. Biol. 7, 2119–2127 (1987).

    Article  CAS  Google Scholar 

  24. Lucchesi, J. C. A. Rev. Genet. 7, 225–237 (1973).

    Article  CAS  Google Scholar 

  25. Poodry, C. A. Drosophila Information Services 55, 210 (1980).

    Google Scholar 

  26. Cramer, L. & Roy, E. Drosophila Information Service 55, 200–204 (1980).

    Google Scholar 

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Authors and Affiliations

  1. Cell Biology Group, the Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, 01545, USA

    Maio S. Chen, Robert A. Obar, Christopher C. Schroeder, Samuel C. Wadsworth & Richard B. Vallee

  2. Department of Biology, University of California, Santa Cruz, California, 95064, USA

    Timothy W. Austin & Clifton A. Poodry

Authors
  1. Maio S. Chen
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  7. Richard B. Vallee
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Chen, M., Obar, R., Schroeder, C. et al. Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis. Nature 351, 583–586 (1991). https://doi.org/10.1038/351583a0

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