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Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs

Nature volume 343, pages 233–238 (1990)Cite this article

Abstract

Microtubules are involved in the transport of vesicles in interphase and of the chromosomes during mitosis. Their arrangement and orientation in the cell are therefore of prime importance and specific patterns are believed to be generated by modulations of the intrinsic dynamic instability of microtubules. Here it is shown that the interphase–metaphase transition of microtubule arrays is under the control of the cdc2 kinase that precisely regulates the dynamics and steady-state length of microtubules.

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References

  1. Wilson, E. B. The Sources of Science 30, 45–87 (Johnson Reprint Corporation, 1966).

    Google Scholar 

  2. Inoue, S. J. Cell Biol. 51, 1315–1475 (1981).

    Google Scholar 

  3. Vorobjev, I. A. & Chentsov Yu. S. J. Cell Biol. 98, 938–949 (1982).

    Article  Google Scholar 

  4. Kuriyama, R. & Borisy, G. G. J. Cell Biol. 91, 822–826 (1981).

    Article  CAS  Google Scholar 

  5. Bajer, A. & Mole-Bajer, J. Chromosoma 27, 448–484 (1969).

    Article  Google Scholar 

  6. Vandre, D. D., Kronebush, P. & Borisy, G. in Molecular Biology of the Cytoskeleton (eds Borisy, G. G., Cleveland, D. W. & Murphy, D. B.) 3–16 (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1984).

    Google Scholar 

  7. Salmon, E. D., Saxton, W. M., Leslie, R. J., Karow, M. L. & McIntosh, R. J. J. Cell Biol. 99, 2157–2164 (1988).

    Article  Google Scholar 

  8. Saxton, W. M. et al. J. Cell Biol. 99, 2175–2186 (1984).

    Article  CAS  Google Scholar 

  9. Nicklas, R. B. Adv. Cell Biol. 2, 225–297 (1971).

    Article  CAS  Google Scholar 

  10. Rieder, C. L. Int. Rev. Cytol. 79, 1–57 (1982).

    Article  CAS  Google Scholar 

  11. Mitchison, T. J. A. Rev. Cell Biol. 4, 527–549 (1988).

    Article  CAS  Google Scholar 

  12. McIntosh, J. R. J. Cell Biol. 83, 428–442 (1979).

    Article  CAS  Google Scholar 

  13. Kirschner, M. & Mitchison, T. Cell 45, 329–342 (1986).

    Article  CAS  Google Scholar 

  14. Karsenti, E., Newport, J., Hubble, R. & Kirschner, M. J. Cell Biol. 98, 1730–1745 (1984).

    Article  CAS  Google Scholar 

  15. Gard, D. L. & Kirschner, M. W. J. Cell Biol. 105, 2191–2201 (1987).

    Article  CAS  Google Scholar 

  16. Gard, D. L. & Kirschner, M. W. J. Cell Biol. 105, 2203–2215 (1987).

    Article  CAS  Google Scholar 

  17. Manes, M. & Barbieri, F. J. embryol. exp. Morph. 40, 187–197 (1977).

    CAS  PubMed  Google Scholar 

  18. Stewart-Savage, J. & Grey, R. D. Wilhelm Roux Arch. dev. Biol. 191, 241–245 (1982).

    Article  CAS  Google Scholar 

  19. Lohka, M. & Maller, J. J. Cell Biol. 101, 518–523 (1985).

    Article  CAS  Google Scholar 

  20. Lhoka, M. F., Hayes, M. K. & Maller, J. L. Proc. natn. Acad. Sci. U.S.A. 85, 3009–3013, (1988).

    Article  ADS  Google Scholar 

  21. Labbé, J. C. et al. Cell 57, 253–263 (1989).

    Article  Google Scholar 

  22. Labbé, J. C. et al EMBO J. 8, 3053–3058 (1989).

    Article  Google Scholar 

  23. Labbé, J. C., Lee, M. G., Nurse, P., Picard, A. & Doree, M. Nature 335, 251–254 (1988).

    Article  ADS  Google Scholar 

  24. Dorée, M., Labbé, J. C. & Picard, A. J. Cell Sci. (in the press).

  25. Felix, M. A., Pines, J., Hunt, T. & Karsenti, E. EMBO J. 8, 3059–3069 (1989).

    Article  CAS  Google Scholar 

  26. Murray, A. & Kirschner, M. Nature 339, 275–280 (1989).

    Article  ADS  CAS  Google Scholar 

  27. Minshull, J., Blow, J. & Hunt, T. Cell 56, 947–956 (1989).

    Article  CAS  Google Scholar 

  28. Gould, K. L. & Nurse, P. Nature 342, 39–45 (1989).

    Article  ADS  CAS  Google Scholar 

  29. Gautier, J., Matsukawa, T., Nurse, P. & Maller, J. Nature 339, 626–629 (1989).

    Article  ADS  CAS  Google Scholar 

  30. Capony, J. P., Picard, A., Peaucellier, G., Labbé, J. C. & Dorée, M. Devl Biol. 117, 1–12 (1986).

    Article  CAS  Google Scholar 

  31. Vandre, D. D., Davis, F. M., Rao, P. T. & Borisy, G. G. Proc. natn. Acad. Sci. U.S.A. 81, 4439–4443 (1984).

    Article  ADS  CAS  Google Scholar 

  32. Vandre, D. D., Davis, F. M., Rao, P. T. & Borisy, G. G. Eur. J. Cell Blol. 41, 72–81 (1986).

    CAS  Google Scholar 

  33. DeBrabander, M. et al. Int. Rev. Cytol. 101, 215–274 (1986).

    Article  CAS  Google Scholar 

  34. Neant, I. & Guerrier, P. Expl Cell Res. 176, 68–79 (1988).

    Article  CAS  Google Scholar 

  35. Neant, I., Charbonneau, M. & Guerrier, P. Devl Biol. 132, 304–314 (1989).

    Article  CAS  Google Scholar 

  36. DeBrabander, M., Guens, G., Nuydens, R., Willebrords, R. & DeMey, J. Cell Biol. int. Rep 5, 913–920 (1981).

    Article  CAS  Google Scholar 

  37. Wadsworth, P. & Salmon, E. D. Cell Motil. 11, 97–105 (1988).

    Article  CAS  Google Scholar 

  38. Hagan, I. M., Hayles, J. & Nurse, P. J. Cell Sci. 91, 587–595 (1988).

    CAS  Google Scholar 

  39. Moreno, S., Hayles, J. & Nurse, P. Cell 58, 361–372 (1989).

    Article  CAS  Google Scholar 

  40. Schulze, E. & Kirschner, M. J. Cell Biol. 102, 1020–1031 (1986).

    Article  CAS  Google Scholar 

  41. Sammak, P. J. & Borisy, G. G. Nature 332, 724–726 (1988).

    Article  ADS  CAS  Google Scholar 

  42. Mitchison, T., Evans, L., Schulze, E. & Kirschner, M. Cell 45, 515–527 (1986).

    Article  CAS  Google Scholar 

  43. Bre, M. H. & Karsenti, E. Cell Motil. (in the press).

  44. Mitchison, T. & Kirschner, M. Nature 312, 232–237 (1984).

    Article  ADS  CAS  Google Scholar 

  45. Walker, R. A. et al. J. Cell Biol. 107, 1437–1448 (1988).

    Article  CAS  Google Scholar 

  46. Bornens, M., Paintrand, M., Berges, J., Marty, M. C. & Karsenti, E. Cell Motil. 8, 238–249 (1987).

    Article  CAS  Google Scholar 

  47. Evans, L., Mitchsion, T. & Kirschner, M. J. Cell Biol. 100, 1185–1191 (1985).

    Article  CAS  Google Scholar 

  48. Laemli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  Google Scholar 

Download references

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Author notes

  1. Jean-Claude Labbé and Marcel Dorée: CNRS and INSERM, BP 5051, 34033 Montpellier Cedex, France

Authors and Affiliations

  1. European Molecular Biology Laboratory, Cell Biology Program, Meyerhofstrasse 1, D-6900, Heidelberg, FRG

    Fulvia Verde, Jean-Claude Labbé, Marcel Dorée & Eric Karsenti

Authors
  1. Fulvia Verde
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  2. Jean-Claude Labbé
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  3. Marcel Dorée
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  4. Eric Karsenti
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Verde, F., Labbé, JC., Dorée, M. et al. Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs. Nature 343, 233–238 (1990). https://doi.org/10.1038/343233a0

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