Abstract
Adenovirus E1A proteins stimulate transcription by RNA polymerases II and III from many promoters1. The detailed mechanism of transcriptional activation (transactivation) by E1A proteins remains unclear, but genetic and biochemical results suggest that E1A products might act to stimulate the activity of cellular transcription factors1. In this study, a detailed mutational analysis of the adenovirus E1B promoter was undertaken to define the DNA sequences required for proper basal transcription and E1A transactivation. Two key findings emerged: first the E1B promoter is an unusually simple RNA polymerase II promoter requiring only two sequence elements for proper regulation, the TATA box2 and a binding site for transcription factor Sp13,4; and second only mutations in the TATA box interfere with E1A-transactivat ion, suggesting that E1A mediates its effect on this promoter through the TATA-box transcription factor.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Berk, A. J. A. Rev. Genet. 20, 45–70 (1986).
Breatnatch, R. & Chambon, P. A. Rev. Biochem. 50, 349–383 (1981).
Dynan, W. W. & Tjian, R. Cell 35, 79–87 (1983).
Kadonaga, J. T., Jones, K. A. & Tjian, R. Trends biochem. Sci. 11, 20–24 (1983).
Kingston, R. E., Kaufman, R. J. & Sharp, P. A. Molec cell. Biol. 4, 1970–1977 (1984).
Leff, T., Corden, J., Elkaim, R. & Sassone-Corsi, P. Nucleic Acids Res. 13, 1209–1221 (1985).
Lewis, E. D. & Manley, J. L. Molec. cell. Biol. 5, 2433–2442 (1985).
Murthy, S. C. S., Bhat, G. P. & Thimmappaya, B. Proc. natn. Acad. Sci. U.S.A. 82, 2230–2234 (1985).
McKnight, S. L. & Kingsbury, R. Science 217, 316–324 (1982).
Zoller, M. J. & Smith, M. Meth. Enzym. 100, 468–501 (1983).
Stow, N. D. J. Virol 37, 171–180 (1981).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Weaver, R. & Wiessman, C. Nucleic Acids Res. 7, 1175–1193 (1979).
Montell, G., Fisher, E. F., Caruthers, M. H. & Berk, A. J. Nature 305, 600–605 (1983).
Galas, D. L. & Schmitz, A. Nucleic Acids Res. 5, 3157–3171 (1978).
Bimstiel, M. L., Busslinger, M. & Strub, K. Cell 41, 349–359 (1985).
McLauchlen, J., Gaffney, D., Witton, J. L. & Clements, J. B. Nucleic Acids Res. 13, 1347–1368 (1985).
Sadofsky, M. & Alwine, J. D. Molec. cell Biol. 4, 1460–1468 (1984).
Hearing, P. & Shenk, T. Cell 33, 695–703 (1983).
Bos, J. L. & ten Wolde-Kraamwinkel, H. C. EMBO J. 2, 73–76 (1983).
Hearing, P. & Shenk, T. Cell 45, 229–236 (1986).
Grosschedl, R. & Bimstiel, M. L. Proc. natn. Acad. Sci. U.S.A. 77, 1432–1436 (1980).
Sawadogo, M. & Roeder, R. Cell 43, 165–175 (1985).
Parker, C. S. & Topol, J. Cell 36, 357–369 (1984).
Green, M. R., Triesman, R. & Maniatis, T. Cell 35, 137–145 (1983).
Zajchowski, D. A., Boeuf, H. & Kedinger, C. EMBO J. 4, 1293–1300 (1985).
Weeks, D. & Jones, N. Nucleic Acids Res. 13, 5389–5402 (1985).
Nevins, J. R. Cell 26, 213–220 (1981).
Osborne, T. F., Arvidson, D. N., Tyau, E. S., Dunsworth-Browne, M. & Berk, A. J. Molec. cell Biol. 4, 1293–1305 (1984).
Montell, C. & Berk, A. J. Cell 36, 951–961 (1984).
Graham, F., Smiley, R. W. & Narin, R. J. gen. Virol. 36, 59–72 (1977).
Jones, N. & Shenk, T. Proc. naln. Acad. Sci. U.S.A. 76, 3665–2669 (1979).
Hirt, B. J. molec. Biol. 26, 365–269 (1967).
Berk, A. J., Lee, F., Harrison, T., William, J. & Sharp, P. A. Cell 17, 935–944 (1979).
Jones, K. A., Yamamoto, K. & Tjian, R. Cell 42, 559–572 (1985).
Kadonaga, J. T. & Tjian, R. Proc. natn. Acad. Sci. U.S.A. 83, 5889–5893 (1986).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wu, L., Rosser, D., Schmidt, M. et al. A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter. Nature 326, 512–515 (1987). https://doi.org/10.1038/326512a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/326512a0
This article is cited by
-
Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin
npj Science of Food (2022)
-
Polycomblike protein PHF1b: a transcriptional sensor for GABA receptor activity
BMC Pharmacology and Toxicology (2013)
-
Deletion analysis of Ad5 E1a transcriptional control region: impact on tumor-selective expression of E1a and E1b
Cancer Gene Therapy (2011)
-
Cilia containing 9 + 2 structures grown from immortalized cells
Cell Research (2007)
-
Immunopathology of human immunodeficiency virus infection in the gastrointestinal tract
Springer Seminars in Immunopathology (1997)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.