Abstract
Recently, we described the presence of a blocking factor (BF) in rat serum which inhibited the histamine release from rat mast cellsin vivo andin vitro. The blocking activity was demonstrated in human serum as well. Purification of the human-BF was carried out in a similar way as previously described for the rodent molecule. Both protein fractions produced a marked suppression of histamine release from rat mast cells and human basophils in a dose-dependent fashion.
Qualitative analysis of the purified preparations demonstrated a major component with a molecular weight of 70 000 daltons. In human serum the blocking factor was identified as transferrin by serological and biochemical methods. It is suggested that this molecule may play an important role in regulating histamine release during allergic and inflammatory reactions.
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Abbreviations
- BBS:
-
borate buffered saline
- BF:
-
blocking factor
- BSA:
-
bovine serum albumin
- DEAE:
-
diethyl-amino-ethyl-cellulose
- DIP:
-
Dactylis inhibitory protein
- IA:
-
immunoaffinity
- IEP:
-
isoelectric point
- kd:
-
kilodalton
- LMB:
-
lymphocytes-monocytes-basophils
- N. b.:
-
Nippostrongylus brasiliensis
- n.d.:
-
not done
- PCA:
-
passive cutaneous anaphylaxis
- RPC:
-
rat peritoneal exudate cells
- SDS:
-
sodium dodecyl sulphate
- TCM:
-
Tris, Ca++, Mg++ buffer
- Tris:
-
Trishydroxy-methyl-amino-methane
References
R. Heatly,The gastrointestinal mast cell, Scand. J. Gastroenterol.18, 449–453 (1983).
J. Bienenstock, A. Befus, F. Pearce, J. Denburg and R. Goodacre,Mast cell heterogeneity: derivation and function with emphasis on the intestine, J. Allergy Clin. Immunol.70, 407–412 (1982).
T. Ishizaka and K. Ishizaka,Activation of mast cells for mediator release through IgE-receptors. InProg. Allergy Vol. 34, pp. 188–235 (Ed. K. Ishizaka), S. Karger, Basel 1984.
R. P. Schleimer, L. M. Lichtenstein and E. Gillespie,Inhibition of basophil histamine release by anti-inflammatory steroids, Nature, London292, 454–455 (1981).
D. Lagunoff, T. Martin and G. Read,Agents that release histamine from mast cells, Ann. Rev. Pharmacol. Toxicol.23, 331–351 (1983).
G. Marone, G. Ambrosio, D. Bonaduce, A. Genovese, M. Triggiani and M. Condorelli,Inhibition of IgE-mediated histamine release from human basophils and mast cells by fenoterol, Int. Archs. Allergy appl. Immunol.74, 356–361 (1984).
S. T. Holgate, R. A. Lewis and K. F. Austen,The role of cyclic nucleotides in mast cell activation and secretion, In:Fougerau, Dausset, Progress in ImmunologyIV, 846–859 (1980).
F. Hirata, W. J. Strittmatter and J. Axelrod, β-adrenergic receptor agonists increase phospholipid methylation, membrane fluidity and β-adrenergic receptor-adenylate cyclase coupling, Proc. Natl. Acad. Sci. USA76.1, 368–372 (1979).
B. Schick, K. F. Austen and L. Schwartz,Activation of rat serosal mast cells by chymase, an endogenous secretory granule protease, J. Immunol.132, 2571–2577 (1984).
T. Ishizaka, D. Conrad, E. Schulman, A. Sterk and K. Ishizaka,Biochemical analysis of initial triggering events of IgE-mediated histamine release from human lung mast cells, J. Immunol.130, 2357–2362 (1983).
D. MacGlashan, R. Schleimer, S. Peters, E. Schulman, K. Adams, A. Sobotka, H. Newball and L. Lichtenstein,Comparative studies of human basophils and mast cells, Fed. Proc.42, 2504–2509 (1983).
T. Ishizaka, A. R. Sterk, M. Daeron, E. L. Becker and K. Ishizaka,Biochemical analysis of desensitization of mouse mast cells, J. Immunol.135, 492–501 (1985).
W. König, P. Pfeiffer and H. W. Kunau,Effect of arachidonic acid metabolites on the histamine release from human basophils and rat mast cells, Int. Arch. Allergy appl. Immunol. suppl.1, 66, 149–151 (1981).
D. L. Marquardt C. W. Parker and T. J. Sullivan,Potentiation of mast cell mediator release by adenosine, J. Immunol.120, 871–878 (1978).
W. König and H. W. Henn,On the latency period of sensitization. Inhibition of passive cutaneous anaphylaxis by a factor in normal rat serum, Int. Archs. Allergy appl. Immunol.58, 182–194 (1979).
K. Theobald and W. König,Characterization of a factor from rat serum with blocking activity in the passive cutaneous anaphylactic reaction, Int. Archs. Aldlergy appl. Immunol.70, 1–11 (1983).
S. Mecheri, G. Peltre and B. David,Induction of a passive cutaneous anaphylaxis inhibitory factor in mice, Int. Archs. Allergy appl. Immunol.78, 290–299 (1985).
B. Ogilvie,Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis, Immunology12, 113–131 (1967).
W. König, M. Baron and F. Ehring,Effect of calcium mineral solutions on the histamine release from human peripheral leukocytes, Drug Res.34, 52–54 (1984).
O. Lowry, N. Rosebrough, A. Farr and R. Randall,Protein measurement with the Folin phenol reagent, J. Biol. Chem.193, 265–275 (1951).
B. Lughtenberg, J. Meijers, R. Peters, P. van der Hoek and L. van Alphen,Electrophoretic resolution of the major outer membrane protein of E. coli K12 into four major bands, FEBS-Letters58, 254–258 (1975).
J. Scheidegger,Une micro-methode de l'immuno-electrophorese, Int. Archs. Allergy appl. Immunol.7, 103–110 (1955).
S. March, I. Parikh and P. Cuatrecasas,A simplified method for cyanogen bromide activation of agarose for affinity chromatography, Anal. Biochem.60, 149–152 (1974).
K. Theobald, W. Gross-Weege, J. Keymling and W. König,Inhibition of histamine release in vitro by a blocking factor from human serum: Comparison with the iron binding proteins transferrin and lactoferrin, Agents and Actions, submitted.
A. Böyum,A one-stage procedure for isolation of granulocytes and lymphocytes from human blood. General sedimentation properties of white blood cells in a 1×g gravity field, Scan. J. Clin. Invest.97, 77–83 (1968).
K. Heide and H. G. Schwick,Salt fractionation of immunoglobulins, InHandbook of Experimental Immunology, Vol. 1 (Ed. D. M. Weir), 7.1–7.11 (1978).
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In partial fulfilment of W. G.-W.'s M.D.-thesis.
Supported by Deutsche Forschungsgemeinschaft (DFG), Kö 427/7-2.
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Groß-Weege, W., Theoblad, K. & König, W. Inhibition of histamine release from rat peritoneal mast cells by a factor from human serum — identification as transferrin. Agents and Actions 19, 10–17 (1986). https://doi.org/10.1007/BF01977250
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DOI: https://doi.org/10.1007/BF01977250