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Phospholipase A2 activation by interleukin 1: Release and metabolism of arachidonic acid by IL 1-stimulated rabbit chondrocytes

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Abstract

Treatment of rabbit chondrocytes with natural or recombinant human IL 1 results in a dramatic dose-dependent increase in intracellular phospholipase A2 (PLA2) activity and subsequent secretion of this enzyme into the intracellular millieu. PLA2 activity is detectable as early as 1 hr after IL 1 stimulation and is maximal by 24 hr. In the present study, we have characterized more fully the relationship between PLA2 activation and arachidonate metabolism in these cells. IL 1 treatment of chondrocytes which had been prelabeled with [14C] arachidonic acid resulted in an enhanced release of free arachidonic acid identified by thin-layer chromatography. Moreover, the arachidonic acid liberated was subsequently metabolized exclusively to PGE2; no significant increases in the production of 6-keto PGF1α, LTB4, LTC4, 12-HETE or 15-HETE were observed following IL 1 stimulation.

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Authors and Affiliations

  1. Immunopharmacology Subdivision, Division of Experimental Therapeutics, Wyeth Laboratories, Inc., P. O. Box 8299, 19101, Philadelphia, PA, USA

    S. C. Gilman Ph. D., P. R. Berner & J. Chang

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  1. S. C. Gilman Ph. D.
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  2. P. R. Berner
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  3. J. Chang
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Gilman, S.C., Berner, P.R. & Chang, J. Phospholipase A2 activation by interleukin 1: Release and metabolism of arachidonic acid by IL 1-stimulated rabbit chondrocytes. Agents and Actions 21, 345–347 (1987). https://doi.org/10.1007/BF01966511

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  • DOI: https://doi.org/10.1007/BF01966511

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