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The possible role of histamine-mediated cAMP formation as a link between H2-receptor stimulation and ultrastructural changes in guinea-pig oxyntic cells

  • Histamine and the Gastrointestinal Tract
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Abstract

Guinea-pig oxyntic cell tubulin has been isolated andin vitro aggregation has been studied. The spontaneous assembly of isolated tubulin was significantly accelerated and increased bt 1 mmol/l GTP. Histamine and forskolin increased tubulin polymerization only when detergent dispersed oxyntic cells or crude membranes were added. The forskolin response occurred very rapidly with an EC50 of approximately 30 μmol/l and did not require GTP. Histamine promoted tubulin aggregation with an EC50 of about 5 μmol/l only in the presence of GTP. Ranitidine completely inhibited the effects of histamine. From these data it is suggested that, in the oxyntic cell, histamine H2-receptor activated adenylate cyclase and the corresponding increase in cAMP play a role in eliciting characteristic ultrastructural changes by initiating formation of microtubules as a first step in the cascade of events leading to an increase in the secretory surface area.

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Authors and Affiliations

  1. Department of Pharmacology, University of Tübingen, Wilhelmstr. 56, D-7400, Tübingen, FRG

    M. Albinus, L. Armbruckner, S. Klein & R. Maier

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  1. M. Albinus
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  2. L. Armbruckner
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  3. S. Klein
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  4. R. Maier
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Albinus, M., Armbruckner, L., Klein, S. et al. The possible role of histamine-mediated cAMP formation as a link between H2-receptor stimulation and ultrastructural changes in guinea-pig oxyntic cells. Agents and Actions 27, 173–176 (1989). https://doi.org/10.1007/BF02222231

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  • DOI: https://doi.org/10.1007/BF02222231

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