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Calcitonin gene-related peptide augments parasympathetic contraction of rabbit tracheal smooth musclein vitro

  • Allergy, Histamine und Kinins
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Abstract

The effect of calcitonin gene-related peptide (CGRP) on parasympathetic contraction of rabbit airway smooth muscle was studied under isometric conditionsin vitro. CGRP (10−7 M) did not contract tracheal smooth muscle, but it potentiated the contractile response to electrical field stimulation (EFS) in a dose-dependent manner, the maximal increase from the baseline response being 14.4±6.2%. In contrast, the contractile responses to exogenously administered acetylcholine were not altered by this peptide. The CGRP-induced potentiation of the contractile responses to EFS was further augmented by physostigmine (123.1%), but not influenced by phentolamine, propranolol, indomethacin, or thiorphan, an enkephalinase inhibitor. These results suggest that CGRP augments the neurally mediated contraction of rabbit airway smooth muscle probably through a prejunctional mechanism and that this effect may not be modulated by tissue enkephalinase.

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Authors and Affiliations

  1. First Department of Medicine, Tokyo Women's Medical College, 8-1, Kawada-Cho, Shinjuku, 162, Tokyo, Japan

    T. Kanemura, J. Tamaoki, S. Horii, N. Sakai, K. Kobayashi, K. Isono, S. Takeuchi & T. Takizawa

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  1. T. Kanemura
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  2. J. Tamaoki
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  3. S. Horii
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  8. T. Takizawa
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Kanemura, T., Tamaoki, J., Horii, S. et al. Calcitonin gene-related peptide augments parasympathetic contraction of rabbit tracheal smooth musclein vitro . Agents and Actions 31, 219–224 (1990). https://doi.org/10.1007/BF01997611

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  • DOI: https://doi.org/10.1007/BF01997611

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