Abstract
The effect of azelastine, an orally effective antiasthmatic/antiallergic drug, on the generation of oxygenderived free radicals in phagocytes was investigated using different chemiluminescence-assays. The chemiluminescence (CL) of both human polymorphonuclear granulocytes (PMNL) and guinea-pig alveolar macrophages (AM) was induced either by phorbol myristate acetate (PMA) or zymosan and amplified either by lucigenin or DMNH (7-dimethylamino-naphthalene-1,2-dicarbonic-acidhydrazide). The inhibitory effect of azelastine was dependent on the inducer employed and the condition and type of cells used. Azelastine reduced PMA-induced CL concentration-dependently in both PMNL (IC30=3.9 μM) and AM (IC30=9.8 μM). In AM zymosan-induced CL was inhibited 21.7% by 10 μM azelastine, whereas in PMNL it remained unchanged up to 10 μM azelastine. Azelastine has a significantly stronger inhibitory effect (IC30=4.2 μM) on oxygen free radical generation in AM primed by fetal calf serum than in unprimed AM. Based on present results it is likely that azelastine inhibitis oxygen-derived free radical generation by interaction with protein kinase C.
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W. Storms, E. Middleton, D. Dvorin, J. Kemp, S. Spector, J. Newton and J. L. Perhach,Azelastine (Azel) in the treatment of asthma. J. Allergy Clin. Immunol.75, 167 (1985).
S. Motojiama, Y. Ohashi T, Otsuka, T. Fukuda and S. Makino,Effects of azelastine on allergen and exercise-induced asthma. Asian Pac. J. Allery3, 174–178 (1985).
S. Ollier, C. A. L. Gould and R. J. Davies,The effect of single and multiple dose therapy with azelastine on the immediate asthmatic response to allergen provocation testing. J. Allergy Clin. Immunol.78, 358–364 (1986).
M. Okuda, T. Ishii, I. Furuuchi, M. Tsutsumi and H. Otsuka,Clinical effects of the oral antiallergic agent E-0659 (azelastine hydrochloride) on allergic rhinitis—double-blind trial with azelastine. Oto-Rhino-Laryngology Tokyo26 [Suppl. 6], 124–163 (1983).
J. F. Connell, J. L. Perhach, J. M. Weiler, R. Rosenthal, L. Hamilton, L. Diamond and J. J. Newton.Azelastine (Az) a new antiallergy agent: efficacy in ragweed hay fever (abst.). Ann. Allergy55, 392 (1985).
H. Magnussen,The inhibitory effect of azelastine and ketotifen on histamine-induced bronchoconstriction in asthmatic patients. Chest91, 855–858 (1987).
I. Szelenyi,Pharmacological profile of azelastine. Drugs of Today25 [Suppl. 6], 3–8 (1989).
M. Honda, K. Miura and T. Tanigawa,Effect of azelastine hydrochloride on macrophage chemotaxis and phagocytosis in vitro. Allergy37, 41–47 (1982).
K. Takanaka,Effect by azelastine on polymorphonuclear leukocytes: Arachidonate cascade inhibition mechanism. Progr. Med.7, 275–278 (1987).
S. R. Durham, M. Carroll, G. M. Walsh and A. B. Kay,Leukocyte activation in allergen-induced late-phase asthmatic reactions. N. Engl. J. Med.311, 1398–1402 (1984).
J. A. Rankin,The contribution of alveolar macrophage to hyperreactive airway disease. J. Allergy Clin. Immunol.83, 722–729 (1989).
J. K. Brieland, R. G. Kunkel and J. C. Fantone,Pulmonary alveolar macrophage function during acute inflammatory lung injury. Am. Rev. Respir. Dis.135, 1300–1306 (1987).
S. Meltzer, B. Goldberg, P. Lad and J. Easton,Superoxide generation and its modulation by adenosine in the neutrophils of subjects with asthma. J. Allergy Clin. Immunol.83, 960–966 (1989).
M. Cluzel, M. Damon, P. Chanez, J. Bousquet, A. Crastes de Paulet, F. B. Michel and Ph. Godard,Enhanced alveolar cell luminol-dependent chemiluminescence in asthma. J. Allergy Clin. Immunol.80, 195–201 (1987).
P. A. Shult, F. M. Grazino and W. W. Busse,Enhanced eosinophil luminol-dependent chemiluminescence in allergic rhinitis. J. Allergy Clin. Immunol.77, 702–708 (1986).
C. Eschenbach and U. Adrian,DMNH—a new sensitive indicator for measuring the chemiluminescence of granulocytes and monocytes. Klin. Wochenschr.63, 1218–1225 (1985).
R. C. Allen,Biochemiexcitation: chemiluminescence and the study of biological oxygenation reactions. InChemical and biological generation of excited states. (Eds. W. Adam and P. Cilento) pp. 309–344. Academic Press, New York 1982.
H. Aniansson, O. Stendahl and C. Dahlgren,Comparison between luminol- and lucigenindependent chemiluminescence of polymorphonuclear leukocytes. Acta path. microbiol. immunol. scand. Sect. C92, 357–361 (1984).
B. M. Babior,The respiratory burst of phagocytes. J. Clin. Invest.73, 599–601 (1984).
H. Gyllenhammar,Lucigenin chemiluminescence in the assessment of neutrophil superoxide production. J. Immunol. Meth.97, 209–213 (1987).
I. Minkenberg and E. Ferber,Lucigenin-dependent chemiluminescence as a new assay for NAD(P)H-oxidase activity in particulate fractions of human polymorphonuclear leukocytes. J. Immunol. Meth.71, 61–67 (1984).
L. R. DeChatelet, G. D. Long, P. S. Shirley, D. A. Bass, M. J. Thomas, F. W. Henderson and M. S. Cohen,Mechanism of the luminol-dependent chemiluminescence of human neutrophils. J. Immunol.129, 1589–1593 (1982).
K. Taniguchi and K. Takanaka,Inhibitory effects of various drugs on phorbol myristate acetate and n-formyl methionyl leucyl phenylalanine induced O −2 production in polymorphonuclear leukocytes. Biochem. Pharmac.33, 3165–3169 (1984).
W. Busse, B. Randlev and J. Sedgwick,The effect of azelastine on neutrophil and eosinophil generation of superoxide. J. Allergy Clin. Immunol.83, 400–405 (1989).
N. O. Christiansen, C. S. Larsen and H. Juhl,Calcium and phorbol ester activation of protein kinase C at intracellular calcium concentration and the effect of TMB-8. Biochim. Biophys. Acta. 882, 57–62 (1986).
I. Maridonneau-Parini, S. M. Tringale and A. I. Tauber,Identification of distinct activation pathways of the human neutrophil NADPH-oxidase. J. Immunol.137, 2925–2929 (1986).
J. E. Smolen, H. M. Korchak and G. Weissman,The roles of extracellular and intracellular Ca ++ in lysosomal enzyme release and superoxide generation by human neutrophils. Biochim. Biophys. Acta.677, 512–520 (1981).
P. L. B. Bruynzeel, L. Koenderman, P. T. M. Kok, M. L. Hameling and J. Verhagen,Platelet-activating factor (PAF-acether) induced leukotriene C 4 formation and luminol dependent chemiluminescence by human eosinophils. Pharm. Res. Commun.18 [Suppl.], 61–69 (1986).
D. W. H. Riches, J. Y. Channon, C. C. Leslie and P. M. Henson,Receptor-mediated signal transduction in mononuclear phagocytes. Prog. Allergy42, 65–122 (1988).
R. I. Sha'afi, J. R. White, T. F. P. Molski, J. Shefcyk, M. Volpi, P. H. Naccache and M. B. Feinstein,Phorbol 12-myristate 13-acetate activates rabbit neutrophils without an apparent rise in the level of intracellular free calcium. Biochim. Biophys. Res. Commun.114, 638–645 (1983).
F. Rossi,The superoxide anion-forming NADPH oxidase of the phagocytes: Nature, mechanism of activation and function. Biochim. Biophys. Acta.853, 65–89 (1986).
R. B. Johnston Jr.,Enhancement of phagocytosis-associated oxidative metabolism as a manifestation of macrophage activation. Lymphokines3, 33–56 (1981).
R. B. Johnston Jr. and S. Kitagawa,Molecular basis for the enhanced respiratory burst of activated macrophages. Federation Proc.44, 2927–2932 (1985).
H. Hayakawa, K. Umehara and Q. N. Myrvik,Oxidative responses of rabbit alveolar macrophages: comparative priming activities of MIF/MAF, sera, and serum components. J. Leuk. Biol.45, 231–238 (1989).
S. Becker,Interferons as modulators of human monocyte-macrophage differentiation. I. Interferon-γ increases HLA-DR expression and inhibits phagocytosis of zymosan. J. Immunol.132, 1249–1254 (1984).
A. Kharazmi, H. Nielsen and K. Bendtzen,Recombinant interleukin 1α and β prime human monocyte superoxide production but have no effect on chemotaxis and oxidative burst response of neutrophils. Immunobiol.177, 32–39 (1988).
I. Szelenyi, U. Achterrath-Tuckermann, J. Schmidt, E. Minker, I. Paegelow and H. Werner,Azelastine: a multifaceted drug for asthma therapy. InNew anti-asthma drugs. (Ed. J. Morley). Agents and Actions [Suppl.] (in press 1990).
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Schmidt, J., Kaufmann, B., Lindstaedt, R. et al. Inhibition of chemiluminescence in granulocytes and alveolar macrophages by azelastine. Agents and Actions 31, 229–236 (1990). https://doi.org/10.1007/BF01997613
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DOI: https://doi.org/10.1007/BF01997613