Chemiluminescence of neutrophil accumulation in liver following ischemia-reperfusion

Abstract

The median and left lateral lobes of rat liverin situ were rendered ischemic for 30 min, then blood flow re-instituted. After 1, 3, 6, 24, or 48 h livers were removed and set up for isolated perfused organ study. Luminol enhanced chemiluminescence (LEC) was recorded from the surface of the median and left lateral lobe before and for 90 min following phorbol myristate acetate PMA (1.6×10⁻⁸ M) perfusion. An increase in PMA induced luminol enhanced chemiluminescence was evident at 1 h, and continued to increase up to 6 h. By 24 h the magnitude of the PMA response had returned to within control values. This indicates that a large influx of inflammatory cells had occurred in the liver following thein vivo ischemia-reperfusion insult, and that these cells gave a very large and sustained burst of radical production on stimulation with PMA.