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The role of non-protein sulfhydryl compounds in gastric adaptive cytoprotection against ethanol-induced mucosal damage in rats

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Abstract

The contribution of the endogenous nonprotein sulfhydryl compounds (SH) in gastric adaptive cytoprotection was investigated in rats. N-ethylmaleimide (NEM) treatment significantly reduced mucosal SH level, and aggravated the mucosal injury induced by absolute ethanol. Oral administration of the mild irritants, 20% ethanol, 5% NaCl or 0.3 M HCl, significantly increased the basal mucosal SH level. These agents also showed a cytoprotective action against the necrotizing effect of absolute ethanol. Administration of NEM did not alleviate this cytoprotective potential, although it abolished the increased SH level evoked by these mild irritants. Thus, it is concluded that modulation of endogenous SH by mild irritants perhaps only plays a minor role in the gastric adaptive cytoprotection.

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References

  1. Robert A. Mechanisms of cytoprotection. In: Paton W, Mitchell J, Turner PP, editors. Proceedings of the 9th International Congress of Pharmacology. New York: Macmillan, 1984;355–9.

    Google Scholar 

  2. Bray TM, Taylor CG. Tissue glutathione, nutrition, and oxidative stress. Can J Physiol Pharmacol 1993;71:746–51.

    PubMed  Google Scholar 

  3. Hiraishi H, Terano A, Ota S, Mutoh H, Sugimoto T, Razandi M, et al. Antioxidant defenses of cultured gastric cells against oxygen metabolites: role of GSH redox cycle and endogenous catalase. Am J Physiol 1991;261:G921–8.

    PubMed  Google Scholar 

  4. Fink R, Clemens MR, Marjot DH, Patsalos P, Cawood P, Norden AG, et al. Increased free-radical activity in alcoholics. Lancet 1985;2:291–4.

    PubMed  Google Scholar 

  5. Jacobson ED. Direct and adaptive cytoprotection. Dig Dis Sci 1986;31:28S–31S.

  6. Robert A, Nezamis JE, Lancaster C, Davis JP, Field SO, Hanchar AJ. Mild irritants prevent gastric necrosis through “adaptive cytoprotection” mediated by prostaglandins. Am J Physiol 1983;245:G113–21.

    PubMed  Google Scholar 

  7. Szabo S, Trier JS, Frankel PW. Sulfhydryl compounds may mediate gastric cytoprotection. Science 1981;214:200–2.

    PubMed  Google Scholar 

  8. Konturek SJ, Brzozowski T, Piastucki I, Radecki T, Dembinski A, Dembinska-Kiec A. Role of locally generated prostaglandins in adaptive gastric cytoprotection. Dig Dis Sci 1982;27:967–71.

    PubMed  Google Scholar 

  9. Cho CH, Pfeiffer CJ, Misra HP. Ulcerogenic mechanism of ethanol and the action of sulphanilyl fluoride on the rat stomach in-vivo. J Pharm Pharmacol 1991;43:495–8.

    PubMed  Google Scholar 

  10. Read SM, Northcote DH. Minimization of variation in the response to different proteins of the Coomassie Blue G-binding assay for protein. Anal Biochem 1981;116:53–8.

    PubMed  Google Scholar 

  11. Hawkey CJ, Kemp RT, Walt RP, Bhaskar NK, Davies J, Filipowicz B. Evidence that adaptive cytoprotection in rats is not mediated by prostaglandins. Gastroenterol 1988;94:948–54.

    PubMed  Google Scholar 

  12. Cho CH, Ko JKS, Tang XL. The differential mechanisms of mild irritants on adaptive cytoprotection. J Gastroenterol Hepatol 1994;9 (Suppl. 1):S24–8.

    PubMed  Google Scholar 

  13. Szabo S, Trier JS. Pathogenesis of acute gastric mucosal injury: sulfhydryl as protector, adrenal cortex as a modulator, and vascular endothelium as a target. In: Flemstrom G, Garner A, Silen W, Turnberg LA, editors. Mechanisms of Mucosal Protection in the Upper Gastrointestinal Tract. New York: Raven Press, 1984:287–93.

    Google Scholar 

  14. Roberts JC, Francetic DJ. The importance of sample preparation and storage in glutathione analysis. Anal Biochem 1993;211:183–7.

    PubMed  Google Scholar 

  15. Cho CH, Fong LYY. The interaction of ethanol and zinc on hepatic glutathione and glutathione transferase activity in mice. Agents Actions 1990;29:382–5.

    PubMed  Google Scholar 

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Ko, J.K.S., Cho, C.H. The role of non-protein sulfhydryl compounds in gastric adaptive cytoprotection against ethanol-induced mucosal damage in rats. Inflamm Res 44, 242–244 (1995). https://doi.org/10.1007/BF01782975

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  • DOI: https://doi.org/10.1007/BF01782975

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