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Effect of hydrogen peroxide on the metabolism of articular chondrocytes

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Abstract.

Objective: To examine the effect of hydrogen peroxide on chondrocyte metabolism.¶Materials and Methods: Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.¶Results: Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.¶Conclusion: Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-higashi 377-2, Osaka-sayama, Osaka 589-8511, Japan, Fax +81 723 660 206, JP

    S. Asada, K. Fukuda, M. Oh, C. Hamanishi & S. Tanaka

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  1. S. Asada
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  2. K. Fukuda
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  3. M. Oh
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  4. C. Hamanishi
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  5. S. Tanaka
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Received 19 August 1998; returned for revision 20 October 1998; accepted by W. B. van den Berg 13 April 1999

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Asada, S., Fukuda, K., Oh, M. et al. Effect of hydrogen peroxide on the metabolism of articular chondrocytes. Inflamm. res. 48, 399–403 (1999). https://doi.org/10.1007/s000110050478

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  • DOI: https://doi.org/10.1007/s000110050478

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