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Regional levels of histamine in rat brain after microwave irradiation: Evidence for artifacts in the enzymatic-isotopic assay

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Abstract

Histamine levels in the brain, measured fluorimetrically, were similar for rats killed by decapitation and microwave irradiation. However, enzymatic-isotopic assay yields 5–20 times higher value for microwave irradiation versus decapitation. Thin layer chromatography of the methylated products in the enzymatic-isotopic assay indicated the formation of artifacts following microwave irradiation. The results strongly suggest that a combination of microwave irradiation and enzymatic-isotopic assay is not suitable for histamine analysis in the rat brain.

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Universität Erlangen-Nürnberg, Universitätsstrasse 22, D-8520, Erlangen, Federal Republic of Germany

    N. Subramanian, W. Schinzel, P. Mitznegg & C. -J. Estler

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  1. N. Subramanian
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  2. W. Schinzel
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  3. P. Mitznegg
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  4. C. -J. Estler
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Subramanian, N., Schinzel, W., Mitznegg, P. et al. Regional levels of histamine in rat brain after microwave irradiation: Evidence for artifacts in the enzymatic-isotopic assay. Agents and Actions 8, 488–490 (1978). https://doi.org/10.1007/BF02111434

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  • DOI: https://doi.org/10.1007/BF02111434

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