Summary
Meiotic and mitotic chromosomes from amphibians and snakes were studied by electron microscopy. By using water spreading, preceded by a mild NaCl pretreatment, we showed: 1. ‘Beads on a string’ arrangement of the chromatin fibres; 2. The presence of loops at pachytene chromomeres as well as during metaphase of both mitosis and first meiosis; 3. Transcriptional activity for non-ribosomal RNA on peripheral loops during the middle pachytene.
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This work was supported by grants from Brazilian National Research Council, CNPq, and Instituto Butantan Research Fund-FEDIB.
We are grateful to Dr R. A. Eckhardt for discussion and suggestions during this investigation and to Dr A. Brunner, Jr, for the permission to use the electron microscope.
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Beçak, M.L., Fukuda, K. & Carneiro, S.M. Chromatin ultrastructure of lower vertebrates. Experientia 33, 1314–1316 (1977). https://doi.org/10.1007/BF01920153
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DOI: https://doi.org/10.1007/BF01920153