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Effect of dimethyl sulfate on the secondary structure of DNA

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Summary

Hydroxyapatite chromatography has been used to demonstrate that alkylation of DNA at neutral pH may lead to denaturation under conditions where no significant depurination occurs. Presence of salt has a preventive effect on such denaturation.

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Authors and Affiliations

  1. Department of Chemistry, Biochemistry Division, Aligarh Muslim University, 202001, Aligarh, (India)

    A. A. Wani,  S. M. Hadi & N. S. Ahmad

Authors
  1. A. A. Wani
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  2. S. M. Hadi
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  3. N. S. Ahmad
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Additional information

Acknowledgment. The authors are grateful to University Grants Commission, India for financial assistance to A. A. W.

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Wani, A.A., Hadi, S.M. & Ahmad, N.S. Effect of dimethyl sulfate on the secondary structure of DNA. Experientia 34, 392–394 (1978). https://doi.org/10.1007/BF01923056

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  • DOI: https://doi.org/10.1007/BF01923056

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