Skip to main content
SpringerLink
Log in

Use of peroxidase-avidin conjugate for the demonstration of intracellular antigen

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Horseradish peroxidase was coupled to avidin by periodate oxidation. Using the peroxidase-avidin conjugate together with biotin-labeled anti-chymotrypsinogen antibody, chymotrypsinogen was clearly demonstrated in the zymogen granules of the pancreas. The reaction between avidin and biotin is so rapid and stable that the avidin-biotin system can be effectively applied to immunohistochemical studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. N.M. Green, Biochem. J.89, 585 (1963).

    Article  CAS  Google Scholar 

  2. H. Heitzmann and F.M. Richards, Proc. natl Acad. Sci. USA71, 3537 (1974).

    Article  CAS  Google Scholar 

  3. E. Skutelsky, Y. Markovsky and D. Danon, Eur. J. Immunol.4, 512 (1974).

    Article  CAS  Google Scholar 

  4. E. Skutelsky, D. Danon, M. Wilchek and E.A. Bayer, J. Ultrastruct. Res.61, 325 (1977).

    Article  CAS  Google Scholar 

  5. E.A. Bayer, E. Skutelsky, D. Wynne and M. Wilchek, J. Histochem. Cytochem.24, 933 (1976).

    Article  CAS  Google Scholar 

  6. E.A. Bayer, M. Wilchek and E. Skutelsky, FEBS Lett.68, 240 (1977).

    Article  Google Scholar 

  7. M.H. Heggeness and J.F. Ash, J. Cell Biol.73, 783 (1977).

    Article  CAS  Google Scholar 

  8. J.L. Guesdon, T. Ternynck and S. Avrameas, J. Histochem. Cytochem.27, 1131 (1979).

    Article  CAS  Google Scholar 

  9. J.M. Becker, M. Wilchek and E. Katchalski, Proc. natl Acad. Sci. USA68, 2604 (1971).

    Article  CAS  Google Scholar 

  10. E. Bayer and M. Wilchek, Meth. Enzymol.34, 265 (1974).

    Article  CAS  Google Scholar 

  11. M.B. Wilson and P.K. Nakane, in: Immunofluorescence and related staining techniques, p. 215. Ed. W. Knapp, K. Holubar and G. Wick. Elsevier/North-Holland Biomedical Press, Amsterdam/New York 1978.

    Google Scholar 

  12. N. Yamamoto, S. Yamashita and K. Yasuda, Acta histochem. cytochem.13, 601 (1980).

    Article  CAS  Google Scholar 

  13. K. Yasuda and A.H. Coons, J. Histochem. Cytochem.14, 303 (1966).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. K. Yasuda

    Present address: Department of Anatomy, School of Medicine, Keio University, Shinanomachi, Shinjuku, Tokyo, Japan

Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Kitasato University, 228, Sagamihara, Kanagawa, Japan

    K. Yasuda, N. Yamamoto & S. Yamashita

Authors
  1. K. Yasuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by Grant-in-Aid for Scientific Research from The Ministry of Education, Science and Culture, The Japanese Government, No. 448086.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yasuda, K., Yamamoto, N. & Yamashita, S. Use of peroxidase-avidin conjugate for the demonstration of intracellular antigen. Experientia 37, 306–308 (1981). https://doi.org/10.1007/BF01991671

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01991671

Keywords

Search

Navigation