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A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and3H-sucrose

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Summary

A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26±0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.

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Authors and Affiliations

  1. Department of Physiology and Pediatrics, Osaka City University Medical School, Abeno-ku, 545, Osaka, Japan

    K. Kageyama, T. Yamashita, T. Kobayashi & E. Kimura

Authors
  1. K. Kageyama
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  2. T. Yamashita
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  3. T. Kobayashi
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  4. E. Kimura
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Kageyama, K., Yamashita, T., Kobayashi, T. et al. A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and3H-sucrose. Experientia 37, 748–750 (1981). https://doi.org/10.1007/BF01967959

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  • DOI: https://doi.org/10.1007/BF01967959

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