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Proteolytic activity on endogeneous substrates in cell-free extracts ofTrypanosoma cruzi

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Summary

Cell-free extracts ofTrypanosoma cruzi contain proteolytic activity able to degrade endogenous substrates. The activity was maximal at pH 3 to 4, had an ‘optimal’ temperature of 65°C, and was strongly inhibited by N-α-p-tosyl-L-lysine chloromethyl ketone.

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Authors and Affiliations

  1. Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas ‘Dr Mario Fatala Chabén’, Ministerio de Salud Pública y Medio Ambiente, Paseo Colón 568, 1063, Buenos Aires, Argentina

    J. J. Cazzulo & Berta M. Franke de Cazzulo

Authors
  1. J. J. Cazzulo
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  2. Berta M. Franke de Cazzulo
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Additional information

This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, the Fundación Julio Cherny, and the Ministerio de Salud Pública y Medio Ambiente de la República Argentina. The authors gratefully acknowledge the technical assistance of Mr Esteban Bontempi.

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Cazzulo, J.J., Franke de Cazzulo, B.M. Proteolytic activity on endogeneous substrates in cell-free extracts ofTrypanosoma cruzi. Experientia 38, 1335–1337 (1982). https://doi.org/10.1007/BF01954936

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  • DOI: https://doi.org/10.1007/BF01954936

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