Summary
Ovariectomized (OVX) rats were injected with various doses of polyestradiol phosphate (PEP); the anterior pituitary (AP) prolactin (PRL) concentration and the plasma afternoon surge of PRL were observed 1 week later by radioimmunoassay. AP PRL was extracted using carbonate and phosphate buffers at either pH 7.6 or 10.6. The AP concentration of PRL was greater when the AP was extracted with buffers at pH 10.6 and the phosphate buffer was the most efficient. The concentration of PRL in the AP more closely reflected the magnitude of the estrogen-induced afternoon surge when the AP was extracted at pH 10.6 and this was especially so when the higher levels of estrogen were administered.
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Supported by NIH Research grant No. HD 14671.
Acknowledgments. The authors would like to express their appreciation to Mrs Mary Romine for art work and to the Rat Pituitary Agency of the National Institute of Arthritis, Metabolism and Digestive Diseases for providing as gifts the rat prolactin-RPI5 and RP-1 used for iodination and standards, respectively, in the rat prolactin radioimmunoassay.
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Gala, R.R., Sensui, N. & Lawson, D.M. Effect of estrogen administration on the induction of the plasma prolactin afternoon surge and on anterior pituitary prolactin concentration extracted at different pHs. Experientia 39, 212–214 (1983). https://doi.org/10.1007/BF01958907
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DOI: https://doi.org/10.1007/BF01958907