Summary
A toxin causing paralysis was detected in the starfishAstropecten latespinosus. The toxin was purified by a method consisting of charcoal treatment and chromatography on CM-Sephadex C-25 and Bio-Rex 70. The toxin was identified as tetrodotoxin by its behavior in thin-layer chromatography and electrophoresis and its1H-NMR spectrum.
References
Hashimoto, Y., Marine toxins and other bioactive marine metabolites. Japan Scientific Societies Press, Tokyo 1979.
Sheumack, D.D., Howden, M.E.H., Spence, I., and Quinn, R.J., Science199 (1978) 188.
Narita, H., Noguchi, T., Maruyama, J., Ueda, Y., Hashimoto, K., Watanabe, Y., and Hida, K., Bull. Jap. Soc. scient. Fish.47 (1981) 935.
Noguchi, T., Maruyama, J., Ueda, Y., Hashimoto, K., and Harada, T., Bull. Jap. Soc. scient. Fish.47 (1981) 909.
Noguchi, T., Maruyama, J., Narita, H., and Hashimoto, K., Toxicon22 (1984) 219.
Noguchi, T., Narita, H., Maruyama, J., and Hashimoto, K., Bull. Jap. Soc. scient. Fish.48 (1982) 1173.
Toxicity was assayed by the official method of determination of TTX using mice. One mouse unit (MU) is defined here as the amount to toxin which kills a 20-g mouse in 30 min on i.p. injection.
Goto, T., Kishi, Y., Takahashi, S., and Hirata, Y., Tetrahedron21 (1965) 2059.
Shoptaugh, N.H., Buckley, L.T., Ikawa, M., and Sasner, J.J. Jr, Toxicon16 (1978) 509.
Tsuda, K., Tachikawa, R., Sakai, K., Tamura, C., Amakasu, O., Kawamura, M., and Ikuma, S., Chem. pharm. Bull., Tokyo12 (1964) 642.
Author information
Authors and Affiliations
Additional information
Acknowledgment. This work was in part supported by a research fund from the Ministry of Agriculture, Forestry and Fisheries of Japan.
Rights and permissions
About this article
Cite this article
Maruyama, J., Noguchi, T., Jeon, J.K. et al. Occurrence of tetrodotoxin in the starfishAstropecten latespinosus. Experientia 40, 1395–1396 (1984). https://doi.org/10.1007/BF01951908
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01951908