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Relationship of prodigiosin condensing enzyme activity to the biosynthesis of prodigiosin and its precursors inSerratia marcescens

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Prodigiosin condensing enzyme (PCE) activities were present inSerratia marcescens wild type 08, mutants OF, WF and 9-3-3. Their specific activities exhibited different maxima and at different times during the late log phase or the early stationary phase of cell growth. The levels of prodigiosin and its precursors also showed a significant increase at this period. The results support that prodigiosin and/or its precursors are secondary metabolites. The ubiquity of the PCE activity in mutants deficient in prodigiosin biosynthesis suggest that this particular enzyme may also be present in non-pigmented clinical isolates.

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References

  1. Qian, H.L., Feng, J.S., and Tsang, J.C., Microbios Lett.33 (1982) 101.

    CAS  Google Scholar 

  2. Qian, H.L., Feng, J.S., Schock, L., Anevski, P., and Tsang J.C., in: Abstracts of the Annual National Meeting of American Society for Microbiology, K-113 (1982).

  3. Loren, J.G., Vinas, M., Fuste, M.C., and Guinea, J., Microbiologica4 (1981) 153.

    Google Scholar 

  4. Hopwood, D.A., Rev. Microbiol.32 (1978) 373.

    Article  CAS  Google Scholar 

  5. Martin, J.F. and Demain, A.L., Microbiol. Rev.44 (1980) 230.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Thompson, C.J., Ward, J.M., and Hopwood, D.A., Cloning of antibiotic resistance and nutritional genes in streptomycetes. J. Bact.151 (1982) 668.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Cai, R.S., Liang, S., Yang, S., Wang, J., and Zhong, J., Nucleic Acids and Proteins. Proc. Symp. Nucleic Acids and Proteins, Beijing, People's Republic of China, p. 581. Van Nostrand Reinhold 1981.

  8. Dauenhauer, S.A., Hull, R.A., and Williams, R.P., J. Bact.158, (1984) 1128.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Holland, S., and Dale, J.W., Microbios Lett.9 (1979) 85.

    Google Scholar 

  10. Muto, Y., Tsuji, A., Kaneoko, Y. and Goto, S., Microbiol. Immun.25 (1981) 1101.

    Article  CAS  Google Scholar 

  11. Platt, D.J., and Summerville, J.S., Microbios Lett.27 (1984) 7.

    CAS  Google Scholar 

  12. Williams, R.P., and Hussain Qadri, S.M., The Pigment ofSerratia. The GenusSerratia, p. 31. Eds A. von Graevenitz and S.J. Rubin. CRC Press, Boca Raton, Florida 1980.

    Google Scholar 

  13. Santer, U.V., PH. D. Thesis, Yale Univ., New Haven 1958.

  14. Morrison, D.A., J. Bact.91 (1966) 1599.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Williams, R.P., and Green, J.A., J. Bact.72 (1956) 537.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Mukherjee, P.P., Goldschmidt, M.E., and Williams, R.P., Biochim. biophys. Acta136 (1967) 182.

    Article  CAS  PubMed  Google Scholar 

  17. Feng, J.S., and Tsang, J.C., in: Abstracts of the Annual National Meeting of American Society for Microbiology, K-11 (1985).

  18. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. biol. Chem.193 (1951) 265.

    Article  CAS  PubMed  Google Scholar 

  19. Wasserman, H.H., McKeon, J.E., Smith, L.A., and Forgione, P., Tetrahedron Suppl.8, Part II (1966) 647.

    Article  Google Scholar 

  20. Williams, R.P., and Gott, C.L., Biochem. biophys. Res. Commun.16 (1964) 47.

    Article  CAS  PubMed  Google Scholar 

  21. Feng, J.S., Qian, H.L., and Tsang, J.C., J. Liquid Chromat.5 (1982) 1329.

    Article  CAS  Google Scholar 

  22. Lowe, J.A., Humphrey, P.E., and Tsang, J.C., Trans. Ill. State Acad. Sci.78 (1985) 121.

    CAS  Google Scholar 

  23. Feitelson, J.S., and Hopwood, D.A., Molec. gen. Genet.190 (1983) 394.

    Article  CAS  PubMed  Google Scholar 

  24. Gerber, N.N., Crit. Rev. Microbiol.3 (1975) 469.

    Article  CAS  Google Scholar 

  25. Patel, K.A., and Dave, P.J., Curr. Sci.49 (1980) 912.

    CAS  Google Scholar 

  26. Patel, K.A., Patel, I.R., Mehta, A.M., and Dave, P.J., Ind. J. exp. Biol.18 (1980) 1353.

    CAS  Google Scholar 

  27. Williams, R.P., Appl. Microbiol.25 (1973) 396.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Weinberg, E.D., Perspect. Biol. Med.14 (1971) 565.

    Article  CAS  PubMed  Google Scholar 

  29. Martin, J.F., Manipulation of gene expression in the development of antibiotic production, in: Antibiotics and Other Secondary Metabolites. Biosynthesis and Production, pp. 19–37. Eds R. Hütter, T. Leisinger, J. Nüesch, and W. Wehrli Academic Press, New York 1978.

    Google Scholar 

  30. Ding, M.J. and Williams, R.P., J. clin. Microbiol.17 (1983) 476.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Cho, L.K.N., Lowe, J.A., Maguire, R.B. et al. Relationship of prodigiosin condensing enzyme activity to the biosynthesis of prodigiosin and its precursors inSerratia marcescens. Experientia 43, 397–399 (1987). https://doi.org/10.1007/BF01940425

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