Skip to main content
SpringerLink
Log in

Zinc antagonizes the effect of botulinum type A toxin at the mouse neuromuscular junction

  • Short Communications
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Zn2+ (10–100 μM) elevated the frequency of miniature end-plate potentials (MEPPs) in the mouse diaphragm. The effect did not depend on external Ca2+. Botulinum type A toxin (BTXA, 50 ng/ml) abolished MEPPs almost completely within 30 min. Zn2+ (100 μM) restored MEPPs and increased their frequency after they had been abolished by BTXA in Ca2+-free solutions. The antagonistic effect of Zn2+ in the Ca2+-free solution was reduced by exposing the diaphragm to the toxin in the Ca2+-free solutions containing high K+. Thus, the action of BTXA is probably enhanced by depolarization of the motor nerve terminals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Simpson, L. L., Pharmac. Rev.33 (1981) 155.

    CAS  Google Scholar 

  2. Boroff, D. A., Del Castillo, J., Evoy, W. H., and Steinhardt, R. A., J. Physiol.240 (1974) 227.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Harris, A. J., and Miledi, R., J. Physiol.217 (1971) 497.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Hirokawa, N., and Heuser, J. E., J. Cell Biol.88 (1981) 160.

    Article  CAS  PubMed  Google Scholar 

  5. Wonnacott, S., Marchbanks, R. M., and Fiol, C., J. Neurochem.30 (1978) 1127.

    Article  CAS  PubMed  Google Scholar 

  6. Cull-Candy, S. G., Lundh, H., and Thesleff, H., J. Physiol.260 (1976) 177.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Kao, I., Deachman, D. B., and Price, D. L., Science193 (1976) 1256.

    Article  CAS  PubMed  Google Scholar 

  8. Pumplin, D. W., and Reese, T. S., J. Physiol.273 (1977) 443.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Lundh, H., and Thesleff, S.., Eur. J. Pharmac.42 (1977) 411.

    Article  CAS  Google Scholar 

  10. Cooper, G. P., Suszkiw, J. B. and Manalis, R. S., in: Cellular and Molecular Neurotoxicology, p. 1. Ed. T. Narahashi, Raven Press, New York 1984.

    Google Scholar 

  11. Kauffman, J. A., Way, J. F. Jr, Siegel, L. S., and Sellin, L. C., Toxic. appl. Pharmac.79 (1985) 211.

    Article  CAS  Google Scholar 

  12. Lundh, H., Leander, S., and Thesleff, S., J. neurol. Sci.32 (1977) 29.

    Article  CAS  PubMed  Google Scholar 

  13. Tse, C. K., Wray, D., Melling, J., and Dolly, J. O., Toxicon24 (1986) 123.

    Article  CAS  PubMed  Google Scholar 

  14. Nishimura, M., J. Physiol.372 (1986) 303.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Nishimura, M., Fujise, N., Yagasaki, O., Kozaki, S., and Sakaguchi, G., Toxic. Lett.31 (suppl.) (1986) 150.

    Google Scholar 

  16. Storey, D. J., Shears, S. B., Kirk, C. J., and Michell, R. H., Nature312 (1984) 374.

    Article  CAS  PubMed  Google Scholar 

  17. Cooke, J. D., Okamoto, K., and Quastel, D. M. J., J. Physiol.228 (1973) 459.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, 591, Sakai, Osaka, Japan

    M. Nishimura, S. Kozaki & G. Sakaguchi

Authors
  1. M. Nishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Kozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Sakaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nishimura, M., Kozaki, S. & Sakaguchi, G. Zinc antagonizes the effect of botulinum type A toxin at the mouse neuromuscular junction. Experientia 44, 18–20 (1988). https://doi.org/10.1007/BF01960227

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01960227

Key words

Key words

Search

Navigation