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Dissociation of digoxin-like immunoreactivity and Na+,K+-ATPase inhibitory activity in rat plasma

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Summary

We measured endogenous digitalis-like factor (EDF) in rat plasma during acute saline infusion by two different procedures. Na+,K+-ATPase inhibitory activity in the rat plasma significantly increased during saline loading (7.8±2.2 vs 2.5±0.9%, with and without acute saline loading, respectively, p<0.05). On the other hand, the plasma digoxin-like immunoreactivity significantly decreased during acute saline loading (16.9±1.6 vs 32.0±2.8 pg digoxin equivalents/ml. with and without acute saline loading, respectively, p<0.01). These results indicate that the major substances detected by digoxin-like immunoreactivity and direct Na+,K+-ATPase inhibitory activity are completely different, at least in rat plasma.

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Authors and Affiliations

  1. Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1- Hongo, Bunkyo-ku, Tokyo 113, Japan

    K. Yamada, A. Goto, M. Ishii & T. Sugimoto

  2. Faculty of Pharmaceutical Science, Setsunan University, Hirakata, Japan

    M. Yoshioka

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  1. K. Yamada
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  2. A. Goto
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  3. M. Ishii
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  4. M. Yoshioka
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  5. T. Sugimoto
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Yamada, K., Goto, A., Ishii, M. et al. Dissociation of digoxin-like immunoreactivity and Na+,K+-ATPase inhibitory activity in rat plasma. Experientia 44, 992–993 (1988). https://doi.org/10.1007/BF01939897

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  • DOI: https://doi.org/10.1007/BF01939897

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