Summary
We measured the effects of recombinant human granulocyte-colony stimulating factor (rhG-CSF) on the adherence of human neutrophils by using a dacron fiber system to assay the adhesive ability of neutrophils. rhG-CSF enhanced neutrophil adherence to dacron fibers. N-formyl-methionyl-leucyl-phenylalanine (fMLP) induced neutrophil-neutrophil interaction (neutrophil aggregation) in addition to neutrophil-dacron interaction, whereas rhG-CSF did not cause neutrophil aggregation. These results indicated that rhG-CSF increases the adhesive ability of neutrophils without neutrophil-neutrophil interaction, and the action of rhG-CSF in neutrophil activation is different from the neutrophil activation caused by fMLP.
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Literature
Metcalf, D., The Haemopoietic Colony Stimulating Factors. Elsevier, Amsterdam 1984.
Metcalf, D., and Nicola, N. A., J. Cell Physiol.116 (1983) 198.
Welte, K., Platzer, E., Lu, L., Gabrilove, J. L., Levi, E., Mertelsmann, R., and Moore, M. A. S., Proc. natl Acad. Sci. USA82 (1985) 1526
Souza, L. M., Boone, T. C. Gabrilove, J., Lai, P. H., Zsebo, K. M., Murdock, D. C., Chazin, V. R., Bruszewski, J., Lu, H., Chen, K. K., Barendt, J., Platzer, E., Moore, M. A. S., Mertelsmann, R., and Welte, K., Science232 (1986) 61.
Morstyn, G., Campbell, L., Souza, L. M., Alton, N. K., Keech, J., Green, M., Sheridan, W., Metcalf, D., and Fox, R., The Lancet1 (1988) 667.
Bronchud, M. H., Potter, M. R., Morgenstern, G., Blasco, M. J., Scarffe, J. H., Thatcher, N., Crowther, D., Souza, L. M., Alton, N. K., Testa, N. G., and Dexter, T. M., Br. J. Cancer58, (1988) 64.
Nicola, N. A., Vadas, M. A., and Lopez, A. F., J. Cell Physiol.128 (1986) 501.
Uzumaki, H., Okabe, T., Sasaki, N., Hagiwara, K., Takaku, F., and Itoh, S., Biochem. biophys. Res. Commun.156 (1989) 1026.
Kitagawa, S., Yuo, A., Souza, L. M., Saito, M., Miura, Y., and Takaku, F., Biochem. biophys. Res. Commun.144 (1987) 1143.
Sullivan, R., Griffin, J. D., Simons, E. R., Schafer, A. I., Meshulam, T., Fredette, J. P., Maas, A. K., Gadenne, A. S., Leavitt, J. L., and Melnick, D. A., J. Immunol.139 (1987) 3422.
Nathan, C. F., Blood73 (1989) 301.
Wang, J. M., Chen, Z. G., Colella, S., Bonilla, M. A., and Welte, K., Blood72 (1988) 1456.
Begley, C. G., Lopez, A. F., Nicola, N. A., Warren, D. J., Vadas, M. A., Sanderson, C. J., and Metcalf, D., Blood68 (1986) 162.
Bronchud, M. H., Scarffe, J. H., Thatcher, N., Crowther, D., Souza, L. M., Alton, N. K., Testa, N. G., and Dexter, T. M., Br. J. Cancer56 (1987) 809.
Cohen, A. M., Hines, D. K., Korach, E. S., and Ratzkin, B. J., Infect. Immun.56 (1988) 2861.
Tanaka, H., Okada, Y., Kawagishi, M., and Tokiwa, T., J. Pharmac. exp. Ther.251 (1989) 1199.
Schwartz, B. R., Ochs, H. D., Beatty, P. G., and Harlan, J. M., Blood65 (1985) 1553.
Schleiffenbaum, B., Moser, R., Patarroyo, M., and Fehr, J., J. Immunol.142 (1989) 3537.
Vadder, N. B., and Harlan, J. M., J. clin. Invest.81 (1988) 676.
Gallin, J. L., and Kaplan, A. P., J. clin. Invest.55 (1975) 967.
Issekutz, A. C., and Ripley, M., Am. J. Hemat.21 (1986) 157.
Ernström, U., and Sandberg, G., Scand. J. Haemat.11 (1973) 275.
Teraoka, T., Hayasaka, Y., Shoji, H., Mineshima, M., Suzuki, T., Sasaki, T., Sato, M., Hoshino, T., Era, K., Tojinbara, T., Nakajima, I., Kawai, T., Honda, H., Fuchinoue, S., Sanaka, T., Agishi, T., and Ota, K., Trans. Am. Soc. artif. intern. Organs34 (1988) 608.
Worthen, G. S., Schwab III, B., Elson, E. L., and Downey, G. P., Science245 (1989) 183.
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Okada, Y., Kawagishi, M. & Kusaka, M. Effect of recombinant human granulocyte colony-stimulating factor on human neutrophil adherence in vitro. Experientia 46, 1050–1053 (1990). https://doi.org/10.1007/BF01940670
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DOI: https://doi.org/10.1007/BF01940670