Skip to main content
SpringerLink
Log in

Increased assembly of cytoskeletal proteins associated with the transformation of human liver cells into fibroblast-like cells

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

Abstract

A topoisomerase II inhibitor, novobiocin, and a deacetylase inhibitor, butyrate, synergistically transformed human liver cells into fibroblast-like cells. This morphological change was associated with an increased production of procollagen type III peptide and a simultaneous assembly of actin, tubulin, vimentin and cytokeratin. Novobiocin and butyrate had no marked effect on the phosphorylation state of cytokeratin proteins, but synergistically enhanced [3H]acetate uptake. From these results, it can be speculated that protein acetylation plays an important role in inducing the assembly of cytoskeletal proteins and the morphological transformation of human liver cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Candido, E. P. M., Reeves, R., and Davie, J. R., Cell14 (1978) 105.

    Article  CAS  PubMed  Google Scholar 

  2. Norton, V. C., Imai, B. S., Yau, D., and Bradbury, E. M., Cell57 (1989) 449.

    Article  CAS  PubMed  Google Scholar 

  3. Han, S., Udvardy, A., and Schedl, P., J. molec. Biol.183 (1985) 13.

    Article  CAS  PubMed  Google Scholar 

  4. Eder, J. P., Teicher, B. A., Holden, S. A., Cathecart, K. N. S., Schnipper, L. E., and Frei, E. III, Cancer Res.49 (1989) 595.

    CAS  PubMed  Google Scholar 

  5. Walters, R. L., and Brizgys, L. M., Cancer Res.48 (1988) 3932.

    Google Scholar 

  6. Kaneko, Y., Nakayama, T., Hamasaki, T., Tsukamoto, A., Toda, G. and Oka, H., Biochem. biophys. Res. Commun.155 (1988) 305.

    Article  CAS  PubMed  Google Scholar 

  7. Boyer, B., Tucker, G. C., Valles, A. M., Franke, W. W., and Thiery, J. P., J. Cell. Biol.109 (1989) 1495.

    Article  CAS  PubMed  Google Scholar 

  8. Sakakibara, K., Igarashi, S., and Hatahara, T., Virchows Arch. path. Anat. Physiol.408 (1985) 219.

    Article  CAS  Google Scholar 

  9. Galambos, S. M. R., Collins, D. C., and Galambos, J. T., Hepatology5 (1985) 38.

    Article  CAS  PubMed  Google Scholar 

  10. Chang, R. S.-M., Proc. Soc. exp. Biol. Med.87 (1954) 440.

    Article  CAS  PubMed  Google Scholar 

  11. Kaneko, Y., Tsukamoto, A., and Kurokawa, K., Cancer Res.51 (1991) 2677.

    CAS  PubMed  Google Scholar 

  12. Kaneko, Y., Biochim. biophys. Acta762 (1983) 111.

    Article  CAS  PubMed  Google Scholar 

  13. Rhode, H., Vargas, L., Haln, E., Kalbfleisch, H., Bruguera, M., and Timpl, M., Eur. J. clin. Invest.9 (1979) 451.

    Article  Google Scholar 

  14. Miyata, Y., Nishida, E., and Sakai, H., Exp. Cell. Res.175 (1988) 280.

    Article  Google Scholar 

  15. Kadowaki, T., Fujita-Yamaguchi, Y., Nishida, E., Takaku, F., Akiyama, T., Kathuria, S., Akanuma, Y., and Kasuga, M., J. biol. Chem.260 (1985) 4016.

    Article  CAS  PubMed  Google Scholar 

  16. Hamaguchi, M., and Hanafusa, H., Proc. natl Acad. Sci. USA84 (1987) 2321.

    Article  Google Scholar 

  17. Yamauchi, T., and Fujisawa, H., Biochem. biophys. Res. Commun.110 (1983) 287.

    Article  CAS  PubMed  Google Scholar 

  18. Lamb, N. J. C., Fernandez, A., Feramisco, J. R., and Welch W. J., J. Cell Biol.108 (1989) 2409.

    Article  CAS  PubMed  Google Scholar 

  19. Inagaki, M., Nishi, Y., Nishizawa, K., Matsuyama, M., and Sato, C., Nature328 (1987) 649.

    Article  CAS  PubMed  Google Scholar 

  20. Piperno, G., LeDizet, M., and Chang, X.-J., J. Cell Biol.104 (1987) 289.

    Article  CAS  PubMed  Google Scholar 

  21. Webb, M. L., Maguyire, K. A., and Jacob, S. T., Nucl. Acids Res.15 (1987) 8547.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Prasad, K., Life Sci.27 (1980) 1351.

    Article  CAS  PubMed  Google Scholar 

  23. Schmid, E., Franke, W. W., Grund, C., Schiller, D. L., Kolb, H., and Paweletz, N., Exp. Cell Res.146 (1983) 309.

    Article  CAS  PubMed  Google Scholar 

  24. Pienta, K. J., Partin, A. W., and Coffey, D. S., Cancer Res.49 (1989) 2525.

    CAS  PubMed  Google Scholar 

  25. Preston, S. F., Volpi, M., Pearson, C. M., and Berlin, R. D., Proc. natl Acad. Sci. USA84 (1987) 5247.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Taniguchi, S., Kawano, T., Kakunaga, T., and Baba, T., J. biol. Chem.261 (1986) 6100.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    A. Tsukamoto, Y. Kaneko & K. Kurokawa

Authors
  1. A. Tsukamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Kaneko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Kurokawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsukamoto, A., Kaneko, Y. & Kurokawa, K. Increased assembly of cytoskeletal proteins associated with the transformation of human liver cells into fibroblast-like cells. Experientia 48, 495–497 (1992). https://doi.org/10.1007/BF01928172

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01928172

Key words

Search

Navigation