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Divergent effects of hyperosmolality on stress-response (heat shock) protein expression in cultured human tumor cells: an immunocytochemical study

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Abstract

Exposing cells to adverse conditions usually elicits expression of stress-response (heat shock) proteins (srp). Here we show that hyperosmolar growth conditions do not uniformly affect srp expression in MCF-7 and HeLa S3 cells, derived from carcinoma of the breast and cervix, respectively. Thus, whereas srp 27 expression was increased in MCF-7, but not in HeLa S3, the opposite was the case with srp 72. On the other hand, hyperosmolality did not induce αB-crystallin or ubiquitin in either cell line. These findings show that srp expression by the human tumor cells studied is non-coordinate, suggesting that each srp is independently modulated.

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Literatur

  1. Ritossa, F., Experientia18 (1962) 571.

    Article  CAS  Google Scholar 

  2. Welch, W. J., Physiol. Rev.72 (1992) 1063.

    Article  CAS  PubMed  Google Scholar 

  3. Morimoto, R. I., Cancer Cells3 (1991) 295.

    CAS  PubMed  Google Scholar 

  4. Ellis, R. J., and van der Vies, S. M., A Rev. Biochem.60 (1991) 321.

    Article  CAS  Google Scholar 

  5. Luo, Y., Amin, J., and Voellmy, R., Molec. cell. Biol.11 (1991) 3660.

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Cohen, D. M., Wasserman, J. C., and Gullans, S. R., Am. J. Physiol.261 (1991) C594.

    Article  CAS  PubMed  Google Scholar 

  7. Soule, H. D., Vasquez, J., Long, A., Albert, S., and Brennan, M., J. natal Cancer Inst.51 (1973) 1409.

    Article  CAS  Google Scholar 

  8. Gey, G. O., Coffman, W. D., and Kubicek, M. T., Cancer Res.12 (1952) 264.

    Google Scholar 

  9. Tandon, A. K., Clark, G. M., Chamness, G. C., Fuqua, S. A. W., Welch, W. J., Riehl, R. M., and McGuire, W. L., Proc. Am. Soc. clin. Oncol.9 (1990) 23.

    Google Scholar 

  10. Thor, A., Benz, C., Moore, D. II, Goldman, E., Edgerton, S., Landry, J., Schwartz, L., Mayall, B., Hickey, E., and Weber, L. A., J. natl. Cancer Inst.83 (1991) 170.

    Article  CAS  PubMed  Google Scholar 

  11. Puy, L. A., Lo Castro, G., Olcese, J. E., Lofti, H. O., Brandy, H. R., and Ciocca, D. R., Cancer64 (1989) 1067.

    Article  CAS  PubMed  Google Scholar 

  12. Iwaki, T., Iwaki, A., Miyazono, M., and, Goldman, J. E., Cancer68 (1991) 2230.

    Article  CAS  PubMed  Google Scholar 

  13. Kato, M., Herz, F., Kato, S., and Hirano, A., Acta neuropath.83 (1992) 420.

    Article  CAS  PubMed  Google Scholar 

  14. Kato, S., Hirano, A., Kato, M., Herz, F., and Ohama, E., Acta neuropath.84 (1992) 261.

    CAS  PubMed  Google Scholar 

  15. Têtu, B., Lacasse, B., Bouchard, H.-L., Lagacé, R., Huot, J., and Landry, J., Cancer Res.52 (1992) 2325.

    PubMed  Google Scholar 

  16. Heufelder, A. E., Goellner, J. R., Wenzel, B. E., and Bahn, R. S., J. clin. Endocr. Metab.74 (1992) 724.

    Article  CAS  PubMed  Google Scholar 

  17. Herz, F., Schermer, A., Halwer, M., and Bogart, L. H., Archs Biochem. Biophys.210 (1981) 581.

    Article  CAS  Google Scholar 

  18. Kato, M., Brijlall, D., Adler, S. A., and Herz, F., Breast Cancer Res. Treat.23 (1992) 241.

    Article  CAS  PubMed  Google Scholar 

  19. Czerniak, B., Herz, F., Wersto, R. P., and Koss, L. G., Proc. natl. Acad. Sci. USA89 (1992) 4860.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Kato, S., Hirano, A., Umahara, T., Kato, M., Herz, F., and Ohama, E., Neuropath. appl. Neurobiol.18 (1992) 335.

    Article  Google Scholar 

  21. Hsu, S.-M., Raine, L., and Fanger, H., J. Histochem. Cytochem.29 (1981) 577.

    Article  CAS  PubMed  Google Scholar 

  22. Towbin, H., Staehelin, T., and Gordon, J., Proc. natl Acad. Sci. USA76 (1979) 4350.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Lee, Y. J., Curetty, L., and Corry, P. M., J. cell. Physiol.149 (1991) 77.

    Article  CAS  PubMed  Google Scholar 

  24. Gupta, R. S., and Singh, B., J. Bact.174 (1992) 4594.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Meury, J., and Kohiyama, M., J. Bact.173 (1991) 4404.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Klemenz, R., Fröhli, E., Steiger, R. H., Schäfer, R., and Aoyama, A., Proc. natl. Acad. Sci. USA88 (1992) 3652.

    Article  Google Scholar 

  27. Inaguma, Y., Shinohara, H., Goto, S., and Kato, K., Biochem. biophys. Res. Commun.182 (1992) 844.

    Article  CAS  PubMed  Google Scholar 

  28. Hickey, E., Brandon, S. E., Potter, R., Stein, J., Stein, G., and Weber, L. A., Nucl. Acids Res.14 (1986) 4127.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Zantema, A., Verlaan-De Vries, M., Maasdam, D., Bol, S., and van der Eb, A., J. biol. Chem.267 (1992) 12936.

    Article  CAS  PubMed  Google Scholar 

  30. Voorter, C. E. M., Wintjes, L., Bloemendal, H., and de Jong, W. W., FEBS Lett.309 (1992) 111.

    Article  CAS  PubMed  Google Scholar 

  31. Dasgupta, S., Hohman, T. C., and Carper, D., Expl. Eye Res.54 (1992) 461.

    Article  CAS  Google Scholar 

  32. Bond, U., and Schlesinger, M. J., Molec. cell. Biol.5 (1985) 949.

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Schlesinger, M. J., J. biol. Chem.265 (1990) 12111.

    Article  CAS  PubMed  Google Scholar 

  34. Petronini, P. G., Alfieri, R., De Angelis, E., Campanini, C., Borghetti, A. F., and Wheeler, K. P., Br. J. Cancer67 (1993) 493.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Petronini, P. G., De Angelis, E. M., Borghetti, A. F., and Wheeler, K. P., Biochem. J.293 (1993) 553.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology Montefiore Medical Center, Albert Einstein College of Medicine, 10467, Bronx, New York, USA

    M. Kato, F. Herz, D. Brijlall & S. Kato

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  1. M. Kato
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  2. F. Herz
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  3. D. Brijlall
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  4. S. Kato
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Kato, M., Herz, F., Brijlall, D. et al. Divergent effects of hyperosmolality on stress-response (heat shock) protein expression in cultured human tumor cells: an immunocytochemical study. Experientia 50, 479–482 (1994). https://doi.org/10.1007/BF01920751

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