Abstract
The uptake ofL-arginine into purified rat brain synaptosomes was investigated with respect to time and various concentrations ofL-[3H] arginine. Specific uptake was found to be linear with time for up to 5 min of incubation at 37°C. Electrolytes, including sodium chloride, potassium chloride, magnesium chloride and calcium chloride, inhibited uptake of 3 μML-arginine, and the inhibitory effect increased with increased electrolyte concentration under constant osmolarity. It was found thatL-arginine was transported into synaptosomes by two uptake components — a high affinity component (3.5 μM) and a low affinity component (100 μM). These two components were similar to the Ly+ system because of their extreme sensitivity to inhibition byL-lysine andL-ornithine but were distinguishable from each other by kinetic analysis of the uptake data and by their relative sensitivity to inhibition by several amino acids.
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Tan, C.H., Ng, F.H. High and low affinity transport ofL-arginine in rat brain synaptosomes. Experientia 51, 1052–1054 (1995). https://doi.org/10.1007/BF01946914
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DOI: https://doi.org/10.1007/BF01946914