Skip to main content
SpringerLink
Log in

A convulsant, 3-mercaptopropionic acid, decreases the level of GABA and GAD in rat pancreatic islets and brain

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

Abstract

To investigate the properties of the gamma-aminobutyric acid (GABA) synthesizing enzyme, glutamate decarboxylase (GAD), in the brain and the pancreatic islets of the rat, GABA concentration in the brain and the pancreatic islets was measured after intraperitoneal administration of 3-mercaptopropionic acid (3-MP) at 25 mg/kg. 60 min after the administration of 3-MP, GABA concentration in the hypothalamus, the superior colliculus and the hippocampus of the brain decreased by 20–30% and in the pancreatic islets by 35%. The concentration in the pancreatic acini did not change. Western blotting showed that GAD activity in the pancreatic islets decreased after administration of 3-MP compared to the control. The activity of GAD in the pancreatic islets as well as brain can be modified by a convulsant, in this case 3-MP. These results suggest the properties of GAD may be similar in the pancreatic islets and brain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Roberts, E., in: GABA in Nervous Systems Function, p. 5. Eds E. Roberts, T. N. Chase and D. E. Power. Raven Press, New York 1976.

    Google Scholar 

  2. Tanaka, C., Life Sci.37 (1985) 2221.

    Article  CAS  PubMed  Google Scholar 

  3. Okada, Y., Taniguchi, H., and Shimada, C., Science195 (1976) 620.

    Article  Google Scholar 

  4. Taniguchi, H., Okada, Y., and Shimada, C., Archs Histol. Jap.40 (1977) 87.

    Article  CAS  Google Scholar 

  5. Baekkeskov, S., Aanstoot, H. J., Christgau S., Reetz, A., Solimena, M., Cascalho, M., Folli, F., Olesen H. R., and Camilli, P. D., Nature347 (1990) 151.

    Article  CAS  PubMed  Google Scholar 

  6. Lamar, C., J. Neurochem.17 (1970) 165.

    Article  CAS  PubMed  Google Scholar 

  7. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem.193 (1951) 265.

    Article  CAS  PubMed  Google Scholar 

  8. Hara, Y., Taniguchi, H., Ishihara, K., Ejiri, K., Tsutou, A., Narutaki, K., and Baba, S., Transplant. Proc.21 (1989) 2632.

    CAS  PubMed  Google Scholar 

  9. Laemmli, U. K., Nature227 (1970) 680.

    Article  CAS  PubMed  Google Scholar 

  10. Loscher, W. Biochem. Pharmac.28 (1979) 1397.

    Article  CAS  Google Scholar 

  11. Okada, Y., Nitsch-Hassler, C., Kim, J. S., Bak, I. J., and Hassler, R., Expl Brain Res.98 (1971) 202.

    Article  Google Scholar 

  12. Cram, D. S., Branett, L. D., Joseph, J. L., and Harrison, L. C., Biochem. biophys. Res. Commun.176 (1991) 1239.

    Article  CAS  PubMed  Google Scholar 

  13. Kaufman, D. L., Hauser, C. R., and Tobin, A. J., J. Neurochem.56 (1991) 720.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Erlander, M. G., Tillakante, N. J. K., Feldbium, S., Patel, N., and Tobin, A. J., Neuron7 (1991) 91.

    Article  CAS  PubMed  Google Scholar 

  15. Erlander, M. G., and Tobin, A. J., Neurochem. Res.16 (1991) 215.

    Article  CAS  PubMed  Google Scholar 

  16. Rorsman, P., Berggren P. O., Bokvist, K., Ericson, H., Mohler, H., Ostensen, C. G., and Smith, P. A., Nature341 (1989) 23.

    Article  Google Scholar 

  17. Taniguchi, H., Okada, Y., Kobayashi, T., Murakami, K., and Baba, S., in: Proinsulin, Insulin and C-peptide, p. 335. Eds S. Baba, T. Kaneko and T. Yanaihara Excepta Medica, Amsterdam 1979.

    Google Scholar 

  18. Zlobina, E. N., Dubinkin, I. V., Merkushov, A. V., Volinskaya, N. A., and Gudima, G. O., Immun. Lett.31 (1992) 289.

    Article  CAS  Google Scholar 

  19. Kaufman, D. L., Erlander, M. J., Salzler, M. C., Atkinson, M. A., Maclaren, M. J., and Tobin, A. J., J. clin. Invest.89 (1992) 283.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Solimena, M., and Camilli, P. D., Trends Neurosci.14 (1991) 452.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Hyogo Rehabilitation Center, Akebono-Cho 1070, 650, Kobe, Japan

    J. Katoh

  2. Second Department of Internal Medicine, Kobe University School of Medicine, 650, Kobe, Japan

    J. Katoh, H. Taniguchi, M. Ogura & M. Kasuga

  3. First Department of Physiology, Kobe University School of Medicine, 650, Kobe, Japan

    Y. Okada

Authors
  1. J. Katoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Taniguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Ogura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Kasuga
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Okada
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Katoh, J., Taniguchi, H., Ogura, M. et al. A convulsant, 3-mercaptopropionic acid, decreases the level of GABA and GAD in rat pancreatic islets and brain. Experientia 51, 217–219 (1995). https://doi.org/10.1007/BF01931099

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01931099

Key words

Search

Navigation