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Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum

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Abstract.

A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.

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Authors and Affiliations

  1. College of Pharmacy, Wonkwang University, Iksan 570-749 (Korea), Fax +82 653 850 7309, e-mail: hslee@wonnms.wonkwang.ac.kr, , , , , , KR

    H. S. Lee, S. B. Kim & B. E. Choi

  2. Drug Targeting Laboratory, College of Pharmacy, Sung Kyun Kwan University, Suwon 440-746 (Korea), , , , , , KR

    Y. S. Kim, B. H. Woo & K. C. Lee

Authors
  1. H. S. Lee
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  2. Y. S. Kim
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  3. S. B. Kim
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  4. B. E. Choi
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  5. B. H. Woo
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  6. K. C. Lee
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Additional information

Received 17 November 1998; received after revision 3 March 1999; accepted 3 March 1999

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Lee, H., Kim, Y., Kim, S. et al. Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum. CMLS, Cell. Mol. Life Sci. 55, 679–682 (1999). https://doi.org/10.1007/s000180050324

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  • DOI: https://doi.org/10.1007/s000180050324

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