Abstract
Blue-green algae are enriched and subcultured in a nitrogen-free medium. Portions of these cultures are kept at 47–48 C for 15, 30, 45 and more min. If akinetes are present and spore-forming bacteria absent — as is often the case — bacteria-free cultures of the algae are obtained.
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References
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Wieringa, K.T. A new method for obtaining bacteria-free cultures of blue-green algae. Antonie van Leeuwenhoek 34, 54–56 (1968). https://doi.org/10.1007/BF02046413
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DOI: https://doi.org/10.1007/BF02046413