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Halotolerance of the yeast Arxula adeninivorans LS3

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Abstract

The non-pathogenic, dimorphic, ascomycetous yeast Arxula adeninivorans LS3 is halotolerant. It can grow in a minimal medium containing up to 20% NaCl. The growth parameters are only weakly influenced by 10% NaCl. However, NaCl in a concentration higher than 10% causes a decrease in the specific growth rate, a longer adaptation phase and a lower cell count in the stationary growth phase. Concentrations of glycerol and trehalose, which differed 100-fold in magnitude in a salt free medium, are also influenced differently by salt. NaCl induces accumulation of intracellular glycerol in exponentially growing cells but a reduced concentration of intracellular trehalose in stationary cells. Transcripts of the genes ARFC3, encoding a component of the replication factor C, and GAA, encoding a secretory glucoamylase, can be detected only in cells cultured in media with NaCl concentrations below 10%. Furthermore, NaCl in high concentration reduces the level of secreted proteins including glucoamylase end invertase.

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Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466, Gatersleben, Germany

    Xiao-Xian Yang, Thomas Wartmann & Regina Stoltenburg

  2. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466, Gatersleben, Germany

    Gotthard Kunze

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  1. Xiao-Xian Yang
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  2. Thomas Wartmann
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  3. Regina Stoltenburg
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  4. Gotthard Kunze
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Correspondence to Gotthard Kunze.

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Yang, XX., Wartmann, T., Stoltenburg, R. et al. Halotolerance of the yeast Arxula adeninivorans LS3. Antonie Van Leeuwenhoek 77, 303–311 (2000). https://doi.org/10.1023/A:1002636606282

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