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Effects ofBacillus thuringiensis var.San diego on predation effectiveness, development and mortality ofColeomegilla maculata lengi (Col.: Coccinellidae) larvae

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Abstract

Laboratory experiments were conducted to determine the effects of M-One™ (Bacillus thuringiensis var.san diego) on larval instars ofColeomegilla maculata lengi Timberlake. Coccinellid larval development (from egg hatch to adult), completed on pollen treated with suspensions of M-One™ at 20 ml/litre (5.6×108 CPBIU/litre) and 200 ml/litre, took respectively 29.3 and 38.5 days compared with 21.9 days for the control (water). M-One™ did not cause larval mortality.C. maculata third instars did not show any preference between eggs ofLeptinotarsa decemlineata (Say) treated with water or with M-One™ at 20 ml/litre. However, at 200 ml M-One™/litre, the number of eggs attacked was 34.7% lower than the eggs treated with water only, 48 h after the beginning of the test. These results indicate that the use of M-One™, at the manufacturer's recommended field rate of 20 ml/litre, does not cause a major threat to larvalC. maculata populations.

Résumé

Des bioessais en laboratoire ont été effectués afin de déterminer si les larves de la coccinelle maculée,Coleomegilla maculata lengi Timberlake (Col.: Coccinellidae), sont affectées par M-One™, un insecticide biologique préparé à partir de la bactérieBacillus thuringiensis var.san diego Berliner et utilisé dans la lutte contre le doryphore de la pomme de terre,Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae). Le développement larvaire, effectué sur du pollen traité avec des concentrations de 20 ml M-One™/litre (5,6×108 unités internationales de doryphore/litre) et 200 ml M-One™/litre, a nécessité 29,3 et 38,5 jours respectivement, comparativement à 21,9 jours pour le témoin (eau). M-One™ n'a pas causé de mortalité chez les larves. Au cours d'un test de 48 h, les larves de stade III n'ont montré aucune préférence entre des œufs traités avec 20 ml M-One™/litre et des œufs traités avec de l'eau. Par contre avec 200 ml M-One™/litre, le nombre d'œufs attaqués a diminué significativement de 34,7% par rapport au témoin, 48 h après le début du test. Ces résultats indiquent que l'utilisation de M-One™ à la concentration recommandée de 20 ml/litre ne représente pas une menace pour les populations larvaires de la coccinelle maculée.

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Authors and Affiliations

  1. Département des Sciences Biologiques, Université du Québec à Montréal, Succ. A, C.P. 8888, H3C 3P8, Montréal, Québec, Canada

    S. Giroux & D. Coderre

  2. Station de recherches, Agriculture Canada 430 boul. Gouin, J3B 3E6, Saint-Jean-sur-Richelieu, Québec, Canada

    C. Vincent & J. -C. Côté

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  1. S. Giroux
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  2. D. Coderre
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  3. C. Vincent
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  4. J. -C. Côté
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Giroux, S., Coderre, D., Vincent, C. et al. Effects ofBacillus thuringiensis var.San diego on predation effectiveness, development and mortality ofColeomegilla maculata lengi (Col.: Coccinellidae) larvae. Entomophaga 39, 61–69 (1994). https://doi.org/10.1007/BF02373495

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