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Heterogeneity of twoBeauveria bassiana strains revealed by biochemical tests, protein profiles and bio-assays ofLeptinotarsa decemlineata (Col.: Chrysomelidae) andColeomegilla macultata lengi (Col.: Coccinellidae) larvae

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Abstract

Biochemical profiles on API Rapid CH* strips and protein profiles on polyacrylamide gels in the presence of sodium dodecyl sulfate were used to distinguish two strains of the entomopathogenic fungusBeauveria bassiana (Balsamo) Vuillemin, ARSEF 2991 and ATCC 44860. Next, the toxicity of these two strains was determined at concentrations of 102, 104, 106 and 108 blastospores/ml on larvae of the Colorado potato beetleLeptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) and of its predator, the spotted ladybird beetle,Coleomegilla maculata lengi Timberlake (Coleoptera: Coccinellidae).

Both strains were highly toxic toL. decemlineata larvae. However, the two strains exhibited different levels of toxicity forC. maculata larvae: ARSEF 2991 was toxic, whereas ATCC 44860 caused little coccinellid larval mortality.

Résumé

Les profils biochimiques sur galeries API Rapid CH* et les profils protéiques sur gels de polyacrylamide ont été utilisés pour distinguer deux souches du champignon entomopathogèneBeauveria bassiana (Balsamo) Vuillemin. La toxicité de ces deux souches a été déterminée à des concentrations de 102, 104, 106 et 108 blastospores/ml sur des larves du doryphore,Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) et de la coccinelle maculéeColeomegilla maculata lengi Timberlake (Coleoptera: Coccinellidae).

Les deux souches deB. bassiana se sont avérées actives à l'égard des larves deL. decemlineata. Toutefois la souche ARSEF 2991 s'est avérée pathogène pour les larves deC. maculata, alors que la souche ATCC 44860 a provoqué une faible mortalité des larves.

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Authors and Affiliations

  1. Département des Sciences Biologiques, Université du Québec à Montréal, Succ. A, C.P. 8888, H3C 3P8, Montréal, Canada

    S. I. Todorova & D. Coderre

  2. Station de Recherches, Agriculture Canada, 430 Boul. Gouin, J3B 3E6, St-Jean-sur-Richelieu, Québec, Canada

    J. -C. Côté & P. Martel

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  1. S. I. Todorova
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  2. J. -C. Côté
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  3. P. Martel
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  4. D. Coderre
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Todorova, S.I., Côté, J.C., Martel, P. et al. Heterogeneity of twoBeauveria bassiana strains revealed by biochemical tests, protein profiles and bio-assays ofLeptinotarsa decemlineata (Col.: Chrysomelidae) andColeomegilla macultata lengi (Col.: Coccinellidae) larvae. Entomophaga 39, 159–169 (1994). https://doi.org/10.1007/BF02372354

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