Summary
In substrate-limited continuous or fed-batch cultures,Clostridium thermolacticum excreted high yield of xylanases even when readily metabolizable compounds such as glucose were used as substrate. These results demonstrated that theC. thermolacticum xylanases were constitutive and were catabolite repressed. Optimization of culture conditions showed that the highest yields were obtained in fed-batch culture.
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References
Antranikian, G., Herzberg, C., and Gottschalk G. (1987).Appl. Environ. Microbiol. 53, 1668–1673.
Antranikian, G., Zablowski, P., and Gottschalk, G. (1987).Appl. Microbiol. Biotechnol. 27, 75–81.
Berenger, J.F., Frixon, C., Bigliardi, J., and Creuset, N. (1985).Can. J. Microbiol. 31, 635–642.
Dekker R.F.H., and Richards, G.N. (1976).Adv. Carbohydr. Chem. Biochem. 32, 277–352.
Debeire, P., Priem, B., Strecker, G. and Vignon, M. (1989).Eur. J. Biochem. in press.
Kidby, D.K., and Davidson, D.J. (1973).Anal. Biochem. 55 321–325.
Royer, J.C., and Nakas, J.P. (1989).Enz. Microbiol. Technol. 11, 405–410.
Wong, K.K.Y., Tan, L.U.L., and Saddler J.N. (1988).Microbiol. Rev. 52, 305–317.
Yu, E.K.C., Tan, L.U.L., Chan, M.H.K., Deschatelets, L., and Saddler, J.N. (1986).Enz. Microbiol. Technol. 9, 16–24.
Zeikus, J.G., and Wolfe, R.S. (1972).J. Bacteriol. 109, 707–713.
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Brodel, B., Samain, E. & Debeire, P. Regulation and optimization of xylanase production inClostridium thermolacticum . Biotechnol Lett 12, 65–70 (1990). https://doi.org/10.1007/BF01028495
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DOI: https://doi.org/10.1007/BF01028495