Summary
Defective interfering particles, present within a high passage inoculum ofAutographa californica nuclear polyhedrosis virus (AcMNPV), interfered with recombinant β-galactosidase and infectious virus production in three insect cell lines. These particles were Selected during serial passage, were missing large parts of the AcMNPV genome, and caused large reductions in β-galactosidase production at multiplicities of infection above 0.01 pfu/cell.
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Wickham, T.J., Davis, T., Granados, R.R. et al. Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection. Biotechnol Lett 13, 483–488 (1991). https://doi.org/10.1007/BF01049204
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DOI: https://doi.org/10.1007/BF01049204