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Continuous hydrolysis ofβ-casein in a membrane reactor: Preparation of a bioactive peptide

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Summary

Continuous Stirred Tank Membrane Reactor was used to investigate the continuous and selective extraction of a bioactive peptideβ-CN (193–209) from bovineβ-casein/chymosin hydrolysate. It was shown that the feasibility of the process depends on the nature and the area of ultrafiltration membrane used. With an inorganic (carbon-zirconia) membrane, high retention of the peptide constitutes a limit to the operation. However, when the reactor was equipped with a cellulosic type membrane, satisfactory transmission of the peptide was obtained. As shown by RP-HPLC and mass spectrometry analysis, onlyβ-CN (193–209) permeates through the membrane.

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Authors and Affiliations

  1. Laboratoire de recherches de technologie laitière, INRA, 65, rue de Saint-Brieuc, 35042, Rennes cedex, France

    S. Bouhallab, D. Mollé & J. Léonil

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  1. S. Bouhallab
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  2. D. Mollé
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  3. J. Léonil
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Bouhallab, S., Mollé, D. & Léonil, J. Continuous hydrolysis ofβ-casein in a membrane reactor: Preparation of a bioactive peptide. Biotechnol Lett 15, 697–702 (1993). https://doi.org/10.1007/BF01080142

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  • DOI: https://doi.org/10.1007/BF01080142

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