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Production of toluene cis-glycol by a recombinant Escherichia coli strain in a two-liquid phase culture system

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Summary

The recombinant Escherichia coli strain, TG2 (p1/1), can be used for biotransformation of toluene to toluene cis-glycol. However, toluene caused substantial inhibition of both growth and toluene cis-glycol production at concentrations above 0.04%. Tetradecane was found to be non-toxic at a phase ratio of 0.23 and provided protection against toluene toxicity. Thus, growth was still possible in the presence of 1.5% toluene. Furthermore, the yield of toluene cis-glycol was increased 1.7-fold compared with the yield in the absence of tetradecane.

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Authors and Affiliations

  1. Department of Chemical Engineering, UMIST, PO Box 88, M60 1QD, Manchester, UK

    J. T. Tsai, L. P. Wahbi, G. A. Dervakos & G. M. Stephens

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  1. J. T. Tsai
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  2. L. P. Wahbi
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  3. G. A. Dervakos
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  4. G. M. Stephens
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Tsai, J.T., Wahbi, L.P., Dervakos, G.A. et al. Production of toluene cis-glycol by a recombinant Escherichia coli strain in a two-liquid phase culture system. Biotechnol Lett 18, 241–244 (1996). https://doi.org/10.1007/BF00142938

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  • DOI: https://doi.org/10.1007/BF00142938

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