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Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labeled DNA probe

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Abstract

A nonisotopic ribosomal DNA fingerprinting technique was developed for the characterization of Listeria monocytogenes. Plasmid pKK3535 (a pBR322-derived plasmid containing rrnB ribosomal RNA operon of Escherichia coli) was labeled with digoxigenin-II-dUTP by random priming and used to probe EcoRI fragments of L. monocytogenes chromosomal DNA on nylon filters. The method was successfully applied to the characterization of two sets of patient and food isolates of L. monocytogenes.

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Authors and Affiliations

  1. Meningitis and Special Pathogens Branch, Division of Bacterial Diseases, Center for Infectious Diseases, Centers for Disease Control, 1-B354 Mail Stop CO2, 30333, Atlanta, Georgia, USA

    L. M. Graves, B. Swaminathan, M. W. Reeves & J. Wenger

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  1. L. M. Graves
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  2. B. Swaminathan
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  3. M. W. Reeves
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  4. J. Wenger
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Graves, L.M., Swaminathan, B., Reeves, M.W. et al. Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labeled DNA probe. Eur J Epidemiol 7, 77–82 (1991). https://doi.org/10.1007/BF00221345

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