Summary
Enzymes in reactive astrocytes of the corebral cortex were examined at the ultrastructural level in an attempt to resolve some conflicting aspects of astrocytic activity. Correlations between morphological and enzyme changes after injury established that the apparent increase in oxidative enzyme activity was exclusively mitochondrial and not an artefactual reaction product resulting from anoxic cellular damage. Pronounced glucose-6-phosphatase activity within cisternae of an increased amount of the granular endoplasnie reticulum was related to increased glycogen. Further evidence from acid phosphatase activity indieated that astrocytes played a minimal role in phagocytosis.
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References
Al-Ali, S. Y. A. (1980)Cytochemistry of rat cerebral cortex and changes in morphology and cytochemistry following injury. Ph.D. Thesis, University of London.
Al-Ali, S. Y. A. &Robinson, N. (1978a) Response of cortical astrocytes to a needle wound seen ultrastructurally.J. Anat. 126, 420.
Al-Ali, S. Y. A. &Robinson, N. (1978b) Histochemical changes in rat neocortex after intracortical needle wound.J. Anat. 127, 205.
Al-Ali, S. Y. A. &Robinson, N. (1979a) Ultrastructural demonstration of cytochrome oxidase via cytochrome c in cerebral cortex.J. Histochem. Cytochem. 27, 1261–6.
Al-Ali, S. Y. A. &Robinson, N. (1979b) Ultrastructural demonstration of dehydrogenases in rat cerebral cortex.Histochemistry 61, 307–18.
Al-Ali, S. Y. A. &Robinson, N. (1981) Ultrastructural demonstration of glucose 6-phosphatase in cerebral cortex.Histochemistry 72, 107–11.
Cook, R. D. &Wisniewski, H. M. (1973) The role of oligodendroglia and astroglia in Wallerian degeneration of the optic nerve.Brain Res. 61, 191–206.
Fujita, H. &Okamoto, H. (1979) Fine structural localization of thiamine pyrophosphatase and acid phosphatase activities in mouse pancreatic acinar cell.Histochemistry 64, 287–295.
Gomori, G. (1952)Microscopic Histochemistry. Chicago: Chicago University Press.
Hirano, A. (1978) Neuronal and glial processes in neuropathology.J. Neuropath. exp. Neurol. 37, 365–74.
Hugon, J. S. &Borgers, M. (1966) Ultrastructural localization of alkaline phosphatase activity in the absorbing cells of the duodenum of mouse.J. Histochem. Cytochem. 14, 629–41.
Klatzo, I. (1967) Neuropathological aspects of brain oedema.J. Neuropath. exp. Neurol. 26, 1–14.
Lantos, P. L. (1977) The distribution and role of microtubules and filaments in the neoplastic astrocytes of experimental glionas.Neuropath App. Neurobiol. 3, 281–96.
Marchesi, V. T. &Palade, G. E. (1967) The localization of Mg−Na−K-activated ATPase on red cell ghost membranes.J. Cell Biol. 35, 385–404.
Matthews, M. A. (1974) Microglia and reactive ‘M’ cells of degenerating central nervous system. Does similar morphology and function imply a common origin?Cell Tiss. Res. 148, 477–91.
Nathaniel, E. J. H. &Nathaniel, D. R. (1978) Degeneration of dorsal roots in the adult rat spinal cord.Expl Neurol. 40, 316–32.
Norenberg, M. D. &Lapham, L. W. (1974) The astrocyte response in experimental portal-systemic encephalopathy. An electron microscopic study.J. Neuropath. exp. Neurol. 33, 422–35.
Novikoff, A. B. &Goldfischer, S. (1961) Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness by cytological studies.Proc. natn. Acad. Sci., U.S.A. 47, 802–10.
Osterberg, K. A. &Wattenberg, I. W. (1962) Oxidative histochemistry of reactive astrocytes.Archs Neurol. 7, 211–8.
Persson, L. (1976) Cellular reaction to small cetebral stab wounds in the rat frontal lobe. An ultrastructural study.Virchows Arch. B. Cell Path. 22, 21–37.
Robinson, N. (1969) Histochemical changes in neocortex and corpus callosum after intracranial injection.J. Neurol. Neurosurg. Psychiat. 32, 317–23.
Robinson, N. (1972) Enzyme response of traumatized tissue after intracortical injection into 5 day old rat brain.J. Neurol. Neurosurg. Psychiat. 35, 865–72.
Schultz, R. L. &Willey, T. J. (1976) Ultrastructure of the sheath around chronically implanted electrodes in brain.J. Neurocytol. 5, 621–4.
Skoff, R. P. (1975) The fine structure of pulse labeled (3H-thymidine cells) in degenerating rat optic nerve.J. comp. Neurol. 161, 595–612.
Smith, B. (1963) Dehydrogenase activity in reactive and neoplastic astrocytes.Brain 86, 89–94.
Torvik, A. (1972) Phagocytosis of nerve cells during retrograde degeneration.J. Neuropath. exp. Neurol. 31, 132–46.
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Al-Ali, S.Y.A., Robinson, N. Ultrastructural study of enzymes in reactive astrocytes: Clarification of astrocytic activity. Histochem J 14, 311–321 (1982). https://doi.org/10.1007/BF01041223
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DOI: https://doi.org/10.1007/BF01041223