Summary
In view of the existence of a different secretion pattern of growth hormone (GH) between male and female rats, the aim of the present study was to analyse the role played by ovarian steroid hormones in the modulation of such secretion. To do so, postpuberal female rats were ovariectomized and killed at 30 days after the operation. The basal serum levels of growth hormone, together with cell area, cytoplasmic area and nuclear area of the hypophyseal somatotropic cells of normal and ovariectomized rats were compared. The results obtained show that ovariectomy induces a significant decrease (p<0.05) in the basal serum levels of GH, accompanied by an increase in cellular and cytoplasmic areas, with no significant differences in nuclear area. Overiectomy was also accompanied by an increase in reaction intensity and the number of GH-immunoreactive cells (p<0.01). These findings point to the shift towards a masculine secretory and morphological pattern following ovariectomy and supports the hypothesis that ovarian steroids intervene in the establishment of a different pattern in females compared to males.
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Akira, S., Wakabayashi, I., Sugihara, H., Minami, S., Takahashi, F. &Moyoyama, A. (1988) Effect of testosterone on growth hormone secretion in female rats during a continuous infusion of growth hormone releasing factor.Neuroendocrinology 47, 116–24.
Blake, C. H. A., Dada, M. O., Olson, D. R. &Campbell, G. T. (1984). A sex difference in the gonadal control of growth hormone secretion in rats.Neuroendocrinol. Lett. 6, 305–10.
Breier, B. H., Gluckman, P. D. &Bass, J. J. (1988). Influence of nutritional status and oestradiol-17 beta on plasma growth hormone, insulin-like growth factors-I and II and the response to exogenous growth hormone in young steers.J. Endocrinol. 118, 243–50.
Carretero, J., Sanchez, F., Montero, M., Sanchez, D., Gonzalez, R. &Vazquez, R. (1988) Estudio morfométrico de las células adenohipofisarias de rata inmunoreactivas a h-GH, tras la administración de Metoclopramida y Met-encefalina.An. Anat. 34, 17–25.
Carretero, J., Sanchez, F., Torres, J. L., Blanco, E., Riesco, J. M. &Vazquez, R. (1989) Study of h-TSH immunoreactive adenohypophyseal cells following treatment with Methymazole.Histol. Histopathol. 4, 71–6.
Dawson-Hughes, B., Stern, D., Goldman, J. &Reichlin, S. (1986). Regulation of growth hormone and somatomedin-C secretion in postmenopausal women: effect of physiological oestrogen replacement.J. Clin. Endocrinol. Metab. 63, 424–32.
Eden, S. (1979). Age and sex related differences in episodic growth hormone secretion in the rat.Endocrinology 105, 555–60.
Eriksson, E. &Jansson, J. O. (1985) Estradiol increases growth hormone secretion in rats exposed to swimming stress or reserpine treatment.Life Sci. 37, 1241–8.
Eriksson, E., Modigh, K. &Jansson, J. O. (1988) Effects of sex steroids on growth hormone responses to clonidine and GHRH in reserpine pretreated rats.J. Neural. Trans. 71, 99–113.
Frohlander, N. &Von-Schoultz, B. (1988) Growth hormone and somatomedin-C during post-menopausal replacement therapy with oestrogen alone and in combination with an antioestrogen.Maturitas 9, 297–302.
Cross, D. S. (1980) Role of somatostatin in the modulation of hypophysial growth hormone production by gonadal steroids.Am. J. Anat. 158, 507–19.
Jansson, J. O., Ekberg, S., Isaksson, O. G. P., Mode, A. &Gustafsson, J. A. (1985) Imprinting of growth hormone secretion, body growth and hepatic steroid metabolism by neonatal testosterone.Endocrinology 117, 1881–9.
Kaplan, L. M., Gabriel, S. M., Koenig, J. I., Sunday, M. E., Spindel, E. R., Martin, J. B. &Chin, W. W. (1988). Galanin is an oestrogen-inducible, secretory product of the rat anterior pituitary.Proc. Natl. Acad. Sci. USA 85, 7408–12.
Martin, J. B., Fukata, J., Millard, W., McCormick, S. &Badger, T. (1985) Pituitary and extrapituitary actions of GRF and somatostain.Folia Endocr. Jap. 61, 231–2.
Millard, W. J., Politch, J. A., Martin, J. B. &Fox, T. O. (1986). Growth hormone-secretory patterns in androgen-resistant (testicular feminized) rats.Endocrinology 119, 2655–60.
Mobbs, C. V., Cheyney, D., Sinha, Y. N. &Finch, C. E. (1985). Age-correlated and ovary-dependent changes in relationship between plasma oestradiol and luteinizing hormone, prolactin and growth hormone in female C57BL/6j mice.Endocrinology 116, 813–20.
Moll, G. W. Jr, Rosenfield, R. L. &Fang, V. S. (1986) Administration of low-dose oestrogen rapidly and directly stimulates growth hormone production.Am. J. Dis. Clild 140, 124–7.
Papas, S., Shin, S. H. &Obonsawin, M. C. (1988) Bovine neurophysin II stimulates growth hormone release in the ostradiol-primed male rat.Neuroendocrinology 47, 154–8.
Sanchez, D. (1988) Análisis de las células GH adenohipofisarias tras la administración de Met-encefalina. Tesis Doctoral en Medicina y Cirugía. Universidad de Salamanca.
Schulte, S. A., Seal, U. S., Plotka, E. D., Verme, L. J., Ozoga, J. J. &Parsons, J. A. (1980) Seasonal changes in prolactin and growth hormone cells in the hypophyses of white-tailed (Odoccoileus virginianus borealis) studied by light microscopic immunocytochemistry and radioimmunoassay.Am. J. Anat. 159, 369–77.
Silverman, B. L., Kaplan, S. L., Grumbach, M. M. &Miller, W. L. (1988) Hormonal regulation of growth hormone secretion and messenger ribonucleic acid accumulation in cultured bovine pituitary cells.Endocrinology 122, 1236–41.
Simard, J., Hubert, J. F., Hosseinzadeh, T. &Labrie, F. (1986) Stimulation of growth hormone release and synthesis by oestrogens in rat anterior pituitary cells in culture.Endocrinology 119, 2004–11.
Simard, J., Vincent, A., Duchesne, R. &Labrie, F. (1988) Full oestrogenic activity of C19-delta 5 adrenal steroids in rat pituitary lactotrophs and somatotrophs.Mol. Cell Endocrinol. 55, 233–42.
Smals, A. E., Pieters, G. F., Smals, A. G., Benraad, T. J., Van-Laarhoven, J. &Kloppenborg, P. W. (1986) Sex difference in human growth hormone (GH) response to intravenous human pancreatic GH-releasing hormone administration in young adults.J. Clin. Endocrinol. Metab. 62, 336–41.
Sternberger, L. A., Hardy, P. H. Jr., Cuculis, J. J. &Meyer, H. G. (1970). The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase anti-horseradish peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem. 18, 315–33.
Streefkerk, J. G. (1972) Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.J. Histochem. Cytochem. 20, 829–31.
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Carretero, J., Sánchez, F., Montero, M. et al. Morphological and functional study of the GH-immunoreactive adenohypophyseal cells in ovariectomized rats. Histochem J 22, 683–687 (1990). https://doi.org/10.1007/BF01047453
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DOI: https://doi.org/10.1007/BF01047453