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Measurement of15N-13C J couplings in staphylococcal nuclease

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Summary

15N-Cα and15N-C′ J couplings were measured for the backbone of staphylococcal nuclease, uniformly enriched with15N and13C. It is found that theIJC'N coupling is similar for β-sheet, J=14.8 ± 0.5 and for α-helix, J = 14.8 ± 0.4 but tends to be larger for the unstructured N- and C-terminal ends of the protein (J=15.6 ± 0.5). On average,1JNCα are smaller for α-helical residues (J=9.6 ± 0.3 Hz) compared to β-sheet (J=10.9 ± 0.8 Hz) and a substantial difference is observed for2JNCα in α-helices (J=6.4 ± 0.4 Hz) and β-sheets (J=8.3 ± 0.8 Hz).

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Authors and Affiliations

  1. Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 20892, Bethesda, MD, USA

    Frank Delaglio & Ad Bax

  2. Bone Research Branch, National Institute of Dental Research, National Institutes of Health, 20892, Bethesda, MD, USA

    Dennis A. Torchia

Authors
  1. Frank Delaglio
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  2. Dennis A. Torchia
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  3. Ad Bax
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Dedicated to the memory of Professor V.F. Bystrov

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Delaglio, F., Torchia, D.A. & Bax, A. Measurement of15N-13C J couplings in staphylococcal nuclease. J Biomol NMR 1, 439–446 (1991). https://doi.org/10.1007/BF02192865

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  • DOI: https://doi.org/10.1007/BF02192865

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