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Determination of the backbone torsion angle ɛ in nucleic acids

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Summary

The multiplet structure of cross peaks in double-quantum-filtered COSY NMR spectra is analysed for those resonances that include passive heteronuclear couplings. Interestingly, the cross peak involving the sugar-ring protons H2′ and H3′ in nucleic acids display an E.COSY-type appearance exclusively when the backbone torsion angle ɛ (C4′-C3′-O3′-P) adopts a gauche(-) conformation. This observation allows an unambiguous analysis of the conformation around ɛ, without the knowledge of 3Jcp coupling constants.

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Author notes

  1. D. Nanz

    Present address: Department of Chemistry, University of California, Berkeley, CA, USA

  2. O. Zerbe

    Present address: Institut für Molekularbiologie und Biophysik, ETH-Hönggerberg, CH-8093, Zürich, Switzerland

Authors and Affiliations

  1. Department of Physics, Ciba-Geigy AG, P.O. Box, CH-4002, Basel, Switzerland

    M. J. J. Blommers

  2. Organisch-chemisches Institut der Universität Zürich, CH-8057, Zürich, Switzerland

    D. Nanz & O. Zerbe

Authors
  1. M. J. J. Blommers
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  2. D. Nanz
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  3. O. Zerbe
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Blommers, M.J.J., Nanz, D. & Zerbe, O. Determination of the backbone torsion angle ɛ in nucleic acids. J Biomol NMR 4, 595–601 (1994). https://doi.org/10.1007/BF00404271

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  • DOI: https://doi.org/10.1007/BF00404271

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