Skip to main content
SpringerLink
Log in

Measurement of three-bond coupling constants in the osmoregulated periplasmic glucan of Burkholderia solanacearum

  • Research Paper
  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

Summary

The cyclic osmoregulated periplasmic glucan produced by Burkholderia solanacearum contains 13 glucose units, all β-(1–2) linked except for one α-(1–6) linkage. We report here the measurement of the 3J(C1-H2′) and 3J(H1-C2′) coupling constants, characterizing the glycosidic linkages, through the use of a 13C/12C double half-filtered NOESY experiment. The values obtained give information about the (Φ, Ψ) angles of the different linkages. The results presented form an important step towards a detailed experimental model of the cyclic glucan, which might allow us to clarify its biological role and establish whether the cavity of these molecules is compatible with the capability of complexing host molecular signals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • André, I., Mazeau, K., Taravel, F.R. and Tvaroska, I. (1995a) Int. J. Biol. Macromol., 17, 189–198.

    Google Scholar 

  • André, I., Mazeau, K., Taravel, F.R. and Tvaroska, I. (1995b) New J. Chem., 19, 331–339.

    Google Scholar 

  • Bax, A. and Summers, M.F. (1986) J. Am. Chem. Soc., 108, 2093–2094.

    Google Scholar 

  • Bax, A. and Ikura, M. (1991) J. Biomol. NMR, 1, 99–104.

    Google Scholar 

  • Breedveld, M.W. and Miller, K.J. (1994) Microbiol. Rev., 58, 145–161.

    Google Scholar 

  • Clore, G.M., Bax, A., Wingfield, P. and Gronenborn, A.M. (1988) FEBS Lett., 238, 17–21.

    Google Scholar 

  • Dowd, M.K., French, A.D. and Reilly, P.J. (1992) Carbohydr. Res., 233, 15–34.

    Google Scholar 

  • Fesik, S.W., Gampe, R.T., Eaton, H.L., Gemmecker, G., Olejniczak, E.T., Neri, P., Holzman, T.F., Egan, D.A., Edalji, R., Simmer, R., Helfrich, R., Hochlowski, J. and Jackson, M. (1991) Biochemistry, 30, 6574–6583.

    Google Scholar 

  • Gottschalk, G. (1986) Bacterial Metabolism, Springer, New York, NY, U.S.A.

    Google Scholar 

  • Harbison, G.S. (1993) J. Am. Chem. Soc., 115, 3026–3027.

    Google Scholar 

  • Ikura, M., Kay, L.E. and Bax, A. (1990) Biochemistry, 29, 4659–4667.

    Google Scholar 

  • Kay, L.E., Ikura, M. and Bax, A. (1991) J. Magn. Reson., 91, 84–92.

    Google Scholar 

  • Lee, W., Harvey, T.S., Yin, Y., Yau, P., Litchfield, D. and Arrowsmith, C.H. (1994) Nature Struct. Biol., 1, 877–890.

    Google Scholar 

  • Lippens, G., Talaga, P., Wieruszeski, J.-M. and Bohin, J.-P. (1995) In Spectroscopy of Biological Molecules (Eds., Merlin, J.C., Turrell, S. and Huvenne, J.-P.), Kluwer, Dordrecht, The Netherlands.

    Google Scholar 

  • Lippens G., Wieruszeski J.-M., Talaga P., Bohin J.-P. and Desvaux H., J. Am. Chem. Soc., 118, 7227–7228.

  • Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989) J. Magn. Reson., 85, 393–399.

    Google Scholar 

  • Montelione, G.T., Winkleer, M.E., Rauenbuehler, P. and Wagner, G. (1989) J. Magn. Reson., 82, 198–204.

    Google Scholar 

  • Otting, G. and Wüthrich, K. (1989) J. Magn. Reson., 85, 586–594.

    Google Scholar 

  • Otting, G. and Wüthrich, K. (1990) Q. Rev. Biophys., 23, 39–96.

    Google Scholar 

  • Poppe, L. and VanHalbeek, H. (1991) J. Magn. Reson., 93, 214–217.

    Google Scholar 

  • Poppe, L., York, W.S. and VanHalbeek, H. (1993) J. Biomol. NMR, 3, 81–89.

    Google Scholar 

  • Serrano, G., Franco-Rodriguez, G., Gonzalez-Jimenez, I., Tejero-Mateo, P., Molina Molina, J., Dobado, J.A., Mégias, M. and Romero, M.J. (1993) J. Mol. Struct., 301, 211–226.

    Google Scholar 

  • Talaga, P., Fournet, B. and Bohin, J.-P. (1994) J. Bacteriol., 176, 6538–6544.

    Google Scholar 

  • Talaga, P., Stahl, B., Wieruszeski, J.-M., Hillenkamp, F., Tsuyumu, S., Lippens, G. and Bohin, J.-P. (1996) J. Bacteriol., 178, 2263–2271.

    Google Scholar 

  • Tvaroska, I., Hricovini, M. and Petrakova, E. (1989) Carbohydr. Res., 189, 359–362.

    Google Scholar 

  • Uhrin, D., Mele, A., Boyd, J., Wormald, M.R. and Dwek, R.A. (1992) J. Magn. Reson., 97, 411–418.

    Google Scholar 

  • Vuister, G. and Bax, A. (1992) J. Magn. Reson., 98, 428–435.

    Google Scholar 

  • Weber, C., Wider, G., VonFreyberg, B., Traber, R., Braun, W., Widmer, H. and Wüthrich, K. (1991) Biochemistry, 30, 6563–6574.

    Google Scholar 

  • Wider, G. and Wüthrich, K. (1993) J. Magn. Reson., B102, 239–241.

    Google Scholar 

  • York, W.S. (1995) Carbohydr. Res., 278, 205–225.

    Google Scholar 

  • Zhang, H., Zhao, D., Revington, M., Lee, W., Jia, X., Arrowsmith, C. and Jardetsky, O. (1994) J. Mol. Biol. 238, 592–614.

    Google Scholar 

Download references

Author information

Author notes

  1. P. Talaga

    Present address: Complex Carbohydrate Research Center, The University of Georgia, 220 Riverbend Road, 30602-4712, Athens, GA, USA

Authors and Affiliations

  1. CNRS URA 1309, Pasteur Institute of Lille, 1, rue du Professeur Calmette, F-59019, Lille, France

    G. Lippens & J. -M. Wieruszeski

  2. CNRS UMR 111, Université des Sciences et Technologies de Lille, F-59655, Villeneuve d'Ascq Cédex, France

    P. Talaga & J. -P. Bohin

Authors
  1. G. Lippens
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. -M. Wieruszeski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Talaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. -P. Bohin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lippens, G., Wieruszeski, J.M., Talaga, P. et al. Measurement of three-bond coupling constants in the osmoregulated periplasmic glucan of Burkholderia solanacearum . J Biomol NMR 8, 311–318 (1996). https://doi.org/10.1007/BF00410329

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00410329

Keywords

Search

Navigation