Skip to main content
SpringerLink
Log in

Monitoring, modeling, and control strategies for expanded-bed adsorption processes

  • Published:
Bioseparation

Abstract

Expanded-bed adsorption (EBA) is a technique for the purification of proteins from cellular debris in downstream processing. An expanded bed presents the possibility of protein recovery in a single step, eliminating the often costly clarification processing steps such as ultrafiltration, centrifugation, and precipitation. An obstacle to the successful commercialization of this technology is the inability to accurately monitor and control the bed height in these systems.

In this paper, we present an overview of work in our laboratory addressing monitoring, modeling, and control strategies as applied to EBA. First, we present the development of a level measurement technique based upon ultrasonics. It is shown that this technique has great promise for bed-height measurement in EBA systems. Second, we present modeling strategies for bed-height dynamics due to flow rate and fluid property changes, and lastly, we show how monitoring and modeling information can be used for the control and regulation of bed expansion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Asif M, Petersen JN, Kaufman EN, Cosgrove JM and Scott TC, (1994) A dynamic model of the hydrodynamics of a liquid fluidized bed. Ind. Eng. Chem. Res., 33: 2151.

    Google Scholar 

  • Bailey JE and Ollis DF (1986) Biochemical Engineering Fundamentals; McGraw-Hill, New York.

    Google Scholar 

  • Campo PJ, and Morari M (1990) Robust control of processes subject to saturation nonlinearities. Computers and Chemical Engineering, 14: 343–358.

    Google Scholar 

  • Chang YK and Chase HA, (1996) Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance. Biotechnol. Bioeng., 49: 512.

    Google Scholar 

  • Chia TL and Lefkowitz I, (1992) Robust PID Tuning Using IMC Technology-Pt. 1, Intech, October: 36.

  • Chien I-L and Fruehauf PS, (1990) Consider IMC Tuning to Improve Controller Performance. Chem. Eng. Prog., October: 33.

  • Coulson JM, Richardson JF, Backhurst JR and Harker JH, (1991) Chemical Engineering: Particle Technology and Separation Processes, 4th ed., Pergamon Press, Oxford.

    Google Scholar 

  • Davis RH, (1995) Velocity of sedimenting particles in suspensions. in: Sedimentation of Small Particles in a Viscous Fluid, Tory EM (ed.) Computational Mechanics Publications, South Hampton, U.K.

    Google Scholar 

  • De Luca L, Hellenbroich D, Titchener-Hooker NJ, Chase HA (1994) A study of the expansion characteristics and transient behaviour of expanded beds of adsorbent particles suitable for bioseparations. Bioseparation, 4: 311–318.

    Google Scholar 

  • Fan L-T, Schmitz JA and Miller EN, (1963) Dynamics of liquidsolid fluidized bed expansion. AIChE J., 9: 149.

    Google Scholar 

  • Fay B (1993) Ultrasonic Backscattering: Fundamentals and Applications. Physical Acoustics: Fundamentals and Applications. In: Oswald L and Breazeale MA, (ed.); Plenum Press: New York.

    Google Scholar 

  • Gailliot FP, Gleason C, Wilson JJ and Zwarick J (1990) Fluidized bed adsorption for whole broth extraction. Biotechnol. Prog. 6: 370–375.

    Google Scholar 

  • Gibilaro LG, Waldram SP, Foscolo PU, (1984) A simple mechanistic description of the unsteady state expansion of liquid fluidized beds. Chem. Eng. Sci. 39: 610.

    Google Scholar 

  • Hjorth R, Kampe S and Carlsson M. (1995) Analysis of some operating parameters of novel adsorbents for recovery of proteins in expanded beds. Bioseparation, 5: 217–223.

    Google Scholar 

  • Kothare MV, Campo PJ, Morari M and Nett, CN, (1994) A unified framework for the study of anti-windup designs. Automatica, 30: 1869–1883.

    Google Scholar 

  • Lee SM (1989) The primary stages of protein recovery. Biotechnol. Prog. 11: 103–118.

    Google Scholar 

  • Marble FE, (1970) Dynamics of dusty gases. Annual Review of Fluid Mechanics 2: 397.

    Google Scholar 

  • Morari M and Zafiriou E (1989) Robust Process Control. Prentice Hall: Englewood Cliffs.

    Google Scholar 

  • Panametrics (1995) Ultrasonic Transducers for Nondestructive Testing, April.

  • Peddieson J Jr (1974) On continuum description of solid-fluid suspensions. Dev. Theor. Appl. Mech., 7: 355.

    Google Scholar 

  • Pharmacia Biotech, (1996) Application Note, STREAMLINE Scale Up, July.

  • Poncelet D, Naveau H, Nyns E-D and Dochain D, (1990) Transient response of a solid-liquid model biological fluidized bed to a step change in fluid superficial velocity. J. Chem. Tech. Biotechnol., 48: 439.

    Google Scholar 

  • Ramirez WF, Shuler PJ and Friedman F, (1980) Convection, dispersion, and adsorption of surfactants in porous media. Soc. Pet. Eng. J., December: 430.

  • Richardson JF and Zaki WN, (1954) Sedimentation and fluidization: Part I. Trans. Instn. Chem. Eng. 32: 35.

    Google Scholar 

  • Rivera DE, Morari M and Skogestad S, (1986) Internal Model Control. 4. PID Controller Design. Ind. Eng. Chem. Process Des. Dev., 25: 252.

    Google Scholar 

  • Seborg DE, Edgar TF, Mellichamp, DA, (1989) Process Dynamics and Control. John Wiley and Sons, New York.

    Google Scholar 

  • Slis PL, Willemse TW and Kramers H (1959) The response of the level of a liquid fluidized bed to a sudden change in the fluidizing velocity. Appl. Sci. Res., 8: 209.

    Google Scholar 

  • Stokes, GG, (1901) Mathematical and Physical Papers.

  • Thelen TV, Mairal AP, Thorsen CS and Ramirez WF (1997) Application of Ultrasonic Backscattering for Level Measurement and Process Monitoring of Expanded-Bed Adsorption Columns. Biotechnol. Prog., 13: 681.

    Google Scholar 

  • Thelen TV and Ramirez, WF, (1997) Bed-height dynamics of expanded beds. Chem. Eng. Sci., 52: 3333.

    Google Scholar 

  • Thelen, TV and Ramirez WF, (1998) Two-Phase Flow Theory for Solid-Liquid Fluidization in the Stokes Flow Regime. AIChE Journal, To appear.

  • Truesdell C (1962) Mechanical basis of diffusion. J. Chem. Phys., 37: 2336.

    Google Scholar 

  • Zafiriou E (1990) Robust Model Predictive Control of Processes with Hard Constraints. Computers and Chemical Engineering, 14: 359–371.

    Google Scholar 

  • Zafiriou E and Morari M (1985) Digital Controllers for SISO systems: A Review and a New Algorithm. Int. J. Control, 42: 855.

    Google Scholar 

  • Zafiriou E and Morari M (1986) Design of robust digital controllers and sampling-time selection for SISO Systems, Int. J. Control, 44: 711.

    Google Scholar 

  • Zhu JJ and Saucier MF, (1992) An IMC-Based Extended PID Controller for Sampled-Data Systems, Automatic Control Conference, 601.

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Colorado, Boulder, CO, 80309-0424, U.S.A

    Travis V. Thelen & W. Fred Ramirez

Authors
  1. Travis V. Thelen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Fred Ramirez
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thelen, T.V., Ramirez, W.F. Monitoring, modeling, and control strategies for expanded-bed adsorption processes. Bioseparation 8, 11–31 (1999). https://doi.org/10.1023/A:1008173007076

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008173007076

Search

Navigation